基于代谢组学研究Cupriavidus necator利用餐厨废油为碳源合成PHB的机理  

MECHANISM OF SYNTHESIZING PHB BY CUPRIAVIDUS NECATOR WITH WASTE COOKING OIL AS THE CARBON SOURCE BASED ON METABOLOMICS ANALYSIS

作　　者：王攀[1] 孙荣 程蒙蒙 任安东 任连海[1] WANG Pan;SUN Rong;CHENG Meng-meng;REN An-dong;REN Lian-hai(School of Light Industry,Beijing Technology and Business University,Beijing 100048,China)

机构地区：[1]北京工商大学轻工科学技术学院

出　　处：《环境工程》2020年第1期151-156,161,共7页Environmental Engineering

基　　金：2018年研究生科研能力提升计划项目;“十三五”时期北京市属高校高水平教师队伍建设支持计划(CIT&TCD201804016);国家自然科学基金(51578008)

摘　　要：运用代谢组学方法研究Cupriavidus necator在不同培养条件下胞内代谢物的变化,寻找影响Cupriavidus necator合成聚-3-羟基丁酸酯(PHB)的生物标志物并探讨代谢机理。利用气相色谱-质谱法(GC/MS)分析不同pH(6、7、8、9)条件下培养Cupriavidus necator的代谢产物,应用偏最小二乘判别分析(PLS-DA)对代谢组学数据进行统计分析,同时结合代谢网络图分析影响PHB合成的代谢途径。PLS-DA分析结果表明:不同pH条件下,鸟氨酸、延胡索酸、β-D-葡萄糖、戊二酸、肌醇、丁酸、甘氨酸、L-天冬酰胺、d-葡萄糖、L-苏氨酸和缬氨酸11种物质表现出显著差异性。结合PLS-DA和代谢途径分析得出:甘氨酸、鸟氨酸、苏氨酸、天冬酰胺和延胡索酸是影响PHB合成的关键代谢物,这些物质通过TCA循环途径、氨基酸合成代谢以及丙酮酸合成降解等途径影响Cupriavidus necator合成PHB。Metabolomics method based on gas chromatography-mass spectrometry(GC-MS)was used to analyze the intracellular metabolites of Cupriavidus necator to obtain potential metabolic biomarkers and reveal the mechanism of poly-3-hydroxybutyrate(PHB)accumulation.The metabolite levels of bacteria under different pH conditions such as 6,7,8 and 9 were investigated.The metabolites data was analyzed by partial least-squares discrimination analysis(PLS-DA),which presented distinct difference between the samples of four different conditions.As the pH changes,11 substances showed significant differences,including ornithine,fumarate,β-D-glucose,glutaric acid,inositol,butyric acid,glycine,L-asparagine,d-glucose,L-threonine and valine.According to PLS-DA and metabolic pathway analysis,glycine,ornithine,threonine,sparagine and fumaric acid were potential metabolic biomarkers.These metabolites influenced PHB production through the metabolic pathways of TCA cycle pathway,amino acid anabolism and pyruvate synthesis degradation.

关 键 词：餐厨废油 Cupriavidus necator 聚-3-羟基丁酸酯 代谢组学 

分 类 号：X799.3[环境科学与工程—环境工程]