艾灸神阙穴对不同程度力竭运动大鼠海马区单胺类神经递质的影响  

Effect of moxibustion on Shenque point on monoamine neurotransmitters in hippocampus of rats with different degrees of exhaustive exercise

作　　者：许晓康[1,2] 周小红 张治方[1] 吕天元 祝婕 高飞[1] 梁玉磊[1,2] 马小顺[1] XU Xiao-kang;ZHOU Xiao-hong;ZHANG Zhi-fang;LV Tian-yuan;ZHU Jie;GAO Fei;LIANG Yu-lei;MA Xiao-shun(Hebei University of Chinese Medicine,Shijiazhuang 050200,China;Hebei Province Laboratory of specific effects of Acupuncture and Moxibustion,Shijiazhuang 050091,China;Hebei Province Traditional Chinese Medicine Hospital,Shijiazhuang 050017,China)

机构地区：[1]河北中医学院,石家庄050200 [2]河北省刺灸法效应特异性重点研究室,石家庄050091 [3]河北省中医院,石家庄050017

出　　处：《中国中医基础医学杂志》2020年第1期41-44,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：河北省教育厅青年自然科学基金项目(QN2016021)-艾灸神阙穴对力竭大鼠中枢单胺类神经递质及自由基代谢影响的研究;河北省中医药管理局科技支撑项目(2017011)-逆灸神阙穴对力竭大鼠中枢单胺类神经递质影响的研究

摘　　要：目的:探讨艾灸神阙穴对不同程度力竭运动大鼠海马区单胺类神经递质的影响。方法:将72只SD雄性大鼠按随机数字表法分为正常组8只、模型组和艾灸组各32只,根据造模或治疗时间将模型组和艾灸组随机分为1、4、7、10次亚组,每亚组8只,采用游泳实验建立力竭模型。艾灸各亚组大鼠造模后即刻温和灸神阙穴15min,隔日1次,各亚组大鼠完成相应力竭运动后24 h检测海马组织5-HT、DA、NE的含量。结果:与正常组比较,模型各亚组5-HT含量均明显升高,模型1、4、7次亚组NE含量均明显升高,模型1次亚组DA含量明显升高,模型10次亚组却明显降低。与相应模型亚组比较,艾灸4次亚组NE含量明显升高,艾灸7、10次亚组5-HT含量显著降低,DA、NE含量明显升高。结论:艾灸神阙穴可以通过调节反复力竭运动大鼠海马区神经递质,促进神经细胞的功能恢复,改善机体疲劳状态,为今后临床应用提供一定的实验基础。Objective:To investigate the effects of moxibustion at“Shénquè”(CV 8)on monoamine neurotransmitters in the hippocampus of rats with different levels of exhaustive exercise.Methods:Seventy-two male SD rats were randomly divided into the following three groups:a control group(n=32)that did not undergo the exercise routine,an untreated group(n=32)that received no treatment after exercise,and a moxibustion group(n=32)that received a mild dose of moxibustion at“Shénquè”(CV 8)for 15min after exercise.Rats in each group were randomly and equally divided into 1 st,4 th,7 th and 10 th trial subgroups according to exercise duration.The exhaustive exercise model was established through repeated trials of swimming.The levels of hippocampal tissue monoamine neurotransmitters 5-hydroxytryptamine(5-HT),Dopamine(DA)and Norepinephrine(NE)were measured after the exhaustive exercise protocol for each group.Results:Compared with the control group,the levels of 5-HT in rat brains for each untreated subgroup were higher,The levels of brain DA in rats from the 1 st trial untreated subgroup were significantly higher than controls and then decreased.The 7 th and 10 th trial untreated subgroups were significantly lower.The levels of brain NE in rats from the 1 st trial untreated subgroup were significantly higher,but its content decreased.Compared with the corresponding untreated subgroups,the levels of rat brain 5-HT in the 7 th and 10 th trial moxibustion subgroups were decreased(P<0.01),the DA levels in the 7 th and 10 th trial moxibustion subgroups were increased,and the NE levels in the 4 th,7 th and 10 th trial moxibustion subgroups were increased.Conclusions:Moxibustion at“Shénquè”(CV 8)could be used to regulate the neurotransmitter content in the rat hippocampus repeatedly to promote the recovery of nerve cells and improve the fatigue state of the body.For the future clinical application of moxibustion at“Shénquè”(CV 8)against exercise fatigue provides a certain experimental basis.

关 键 词：神阙穴 力竭运动 海马组织 5-HT DA NE 

分 类 号：R245.81[医药卫生—针灸推拿学]