基于大鼠离体外翻肠囊模型研究不同粒径天麻粉的吸收特性  

Study on Absorption Characteristics of Gastrodia elata Powder with Different Particle Sizes Based on Rat Everted Intestinal Sac Model in vitro

作　　者：陈艳[1] 刘帆[1] 巩仔鹏[1] 陈亭亭 陶陶[2] 刘智 王爱民[1] CHEN Yan;LIU Fan;GONG Zipeng;CHEN Tingting;TAO Tao;LIU Zhi;WANG Aimin(Guizhou Provincial Key Laboratory of Pharmaceutics&State Key Laboratory of Functions and Applications of Medicinal Plants&Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and TCM&School of Pharmacy,Guizhou Medical University,Guiyang 550004,China;Dept.of Rehabilitation Medicine,Guizhou Provincial People’s Hospital,Guiyang 550002,China;Dept.of Pharmacy,the Affiliated Hospital of Guizhou Medical University,Guiyang 550001,China)

机构地区：[1]贵州医科大学贵州省药物制剂重点实验室/省部共建药用植物功效与利用国家重点实验室/民族药与中药开发应用教育部工程研究中心/药学院,贵阳550004 [2]贵州省人民医院康复医学科,贵阳550002 [3]贵州医科大学附属医院药剂科,贵阳550001

出　　处：《中国药房》2020年第4期413-422,共10页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81560646,No.U1812403);中央引导地方科技发展专项资金项目(No.黔科中引地[2018]4006);贵州省科技计划项目(No.黔科合平台人才[2016]5613,No.黔科合平台人才[2016]5677);贵阳市科技计划项目(No.筑科合同[2017]30-29号)

摘　　要：目的:比较不同粒径天麻粉中天麻素、巴利森苷A、巴利森苷B、巴利森苷C的肠吸收特征,探讨粒径对上述成分肠吸收的影响。方法:采用大鼠外翻肠囊模型,以累积吸收量(Q)和吸收速率常数(Ka)为指标,采用超高效液相色谱-串联质谱法测定不同剂量(2.5、5、10 g/L)和不同粒径(细粉146μm、极细粉52μm、超微粉37μm)的天麻粉中天麻素、巴利森苷A、巴利森苷B、巴利森苷C在不同肠段(十二指肠、空肠、回肠、结肠)中的吸收情况。结果:2.5 g/L天麻极细粉中天麻素和巴利森苷B的Q、Ka值(全肠段),巴利森苷C的Q值(结肠)和Ka值(回肠、结肠);2.5 g/L天麻超微粉中天麻素的Q、Ka值(全肠段),巴利森苷B的Q、Ka值(十二指肠、空肠、回肠),巴利森苷C的Ka值(结肠);5 g/L天麻极细粉中天麻素的Q值(十二指肠),巴利森苷A和巴利森苷B的Q值(全肠段),巴利森苷C的Q值(十二指肠、空肠);5 g/L天麻超微粉中天麻素的Q值(十二指肠、空肠、结肠)和Ka值(全肠段),巴利森苷B的Q值(十二指肠、回肠、结肠),巴利森苷C的Q值(十二指肠、回肠);10 g/L天麻极细粉中巴利森苷B的Q、Ka值(空肠、回肠),巴利森苷C的Q值(空肠、回肠)以及10 g/L天麻超微粉中天麻素的Q值(结肠)和Ka值(十二指肠),巴利森苷B的Q值(十二指肠、回肠、结肠)和Ka值(十二指肠、结肠),巴利森苷C的Q值(十二指肠、回肠)和Ka值(十二指肠)均较同剂量天麻细粉显著升高(P<0.05或P<0.01)。2.5 g/L天麻极细粉中巴利森苷A的Ka值(空肠),巴利森苷C的Q值(十二指肠);2.5 g/L天麻超微粉中巴利森苷A的Ka值(空肠、回肠),巴利森苷C的Q、Ka值(十二指肠、空肠);5 g/L天麻极细粉中天麻素的Ka值(空肠、回肠、结肠),巴利森苷A的Ka值(结肠),巴利森苷B的Ka值(回肠),巴利森苷C的Ka值(空肠、回肠);5 g/L天麻超微粉中天麻素和巴利森苷C的Ka值(空肠、回肠、结肠),巴利森苷A的Q值(空肠、结肠)和Ka值(OBJECTIVE:To compare the absorption characteristics of gastrodin,parishin A,parishin B and parishin C of Gastrodia elata powder,and to explore the effect of particle size on intestinal absorption of above components.METHODS:Based on everted intestinal sac model,using accumulative absorption amount(Q)and absorption rate constant(Ka)as indexes,UPLC-MS/MS method was used to determine the absorption of gastrodin,parishin A,parishin B and parishin C from different doses(2.5,5,10 g/L)of G.elata powder with different particle sizes(fine powder 146μm,superfine powder 52μm,ultrafine powder 37μm)in different segments(duodenum,jejunum,ileum and colon).RESULTS:Q and Ka of gastrodin and parishin B(intestinal segment),Q(colon)and Ka(ileum and colon)of parishin C in 2.5 g/L G.elata superfine powder;Q and Ka of gastrodin(intestinal segment),Q and Ka of parishin B(duodenum,jejunum,ileum)and Ka of parishin C(colon)in 2.5 g/L G.elata ultrafine powder;Q of gastrodin(duodenum),Q of parishin A and parishin B(intestinal segment)and Q of parishin C(duodenum,jejunum)in 5 g/L G.elata superfine powder;Q(duodenum jejunum,colon)and Ka(intestinal segment)of gastrodin,Q of parishin B(duodenum,ileum and colon)and Q of parishin C(duodenum,ileum)in 5 g/L G.elata ultrafine powder;Q and Ka of parishin B(jejunum,ileum),Q of parishin C(jejunum,ileum)in 10 g/L G.elata superfine powder as well as Q(colon)and Ka(duodenum)of gastrodin,Q(duodenum,ileum,colon)and Ka(duodenum,colon)of parishin B,Q(duodenum,ileum)and Ka(duodenum)of parishin C in 10 g/L G.elata ultrafine powder were all increased significantly,compared with the same dose of G.elata fine powder(P<0.05 or P<0.01).Ka of parishin A(jejunum)and Q of parishin C(duodenum)in 2.5 g/L G.elata superfine powder;Ka of parishin A(jejunum,ileum),Q and Ka of parishin C(duodenum,jejunum)in 2.5 g/L G.elata ultrafine powder;Ka of gastrodin(jejunum,ileum and colon),Ka of parishin A(colon),Ka of parishin B(ileum)and Ka of parishin C(jejunum,ileum)in 5 g/L G.elata superfine powder;Ka of gastrodin and parishin C

关 键 词：天麻 粒径 外翻肠囊模型 吸收特性 天麻素 巴利森苷类 

分 类 号：R971[医药卫生—药品] R969.1[医药卫生—药学]