二苯乙烯苷和远志总皂苷配伍对Aβ25-35诱导PC12细胞损伤的影响  

Experimental Study on the Effects of Combination of Tetrahydroxy Stilbene Glycoside and Tenuigenin on PC12 Cell Injury Induced by Aβ25-3

作　　者：张运辉[1,2] 周小青 伍大华[3] 杨梦琳[1,2] 郑彩杏[1] 童天昊 张祺杰 ZHANG Yunhui;ZHOU Xiaoqing;WU Dahua;YANG Menglin;ZHENG Caixing;TONG Tianhao;ZHANG Qijie(Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;School of Chinese Medicine,Chongqing Three Gorges Medical College,Chongqing 404120,China;Hunan Academy of Chinese Medicine,Changsha,Hunan 410006,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]重庆三峡医药高等专科学校中医学院,重庆404120 [3]湖南省中医药研究院,湖南长沙410006

出　　处：《湖南中医药大学学报》2020年第2期134-138,共5页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金资助项目（81373720）

摘　　要：目的探讨二苯乙烯苷(tetrahydroxy stilbene glycoside,TSG)和远志总皂苷(Tenuigenin,TEN)配伍对Aβ25-35诱导的PC12细胞损伤的影响。方法PC12细胞除空白组外运用Aβ25-35诱导建立阿尔茨海默病(Alzheimer’s disease,AD)模型,被随机分成模型组、TSG(200 mmol/L)组、TEN组(100 mg/L)、TSG-TEN配伍组(TSG200 mmol/L+TEN100 mg/L)、多奈哌齐组(10μmol/L)。采用MTT比色法检测各组细胞存活率;取细胞上清液分别测各组的乳酸脱氢酶(LDH)、丙二醛(MDA)含量及乙酰胆碱酯酶(AchE)、超氧化物歧化酶(SOD)活力;统计各组的细胞分化率。结果(1)细胞存活率的高低为:TSG-TEN组>TSG组>多奈哌齐组>TEN组(P<0.01);TSG组、TEN组、多奈哌齐组均低于TSG-TEN配伍组(P<0.01,P<0.05);(2)与模型组比较,TSG组、TEN组、TSG-TEN配伍组可降低LDH含量、MDA含量、抑制AchE活力及增强SOD活力(P<0.05)。且TSG-TEN配伍组对降低LDH含量、MDA含量、抑制AchE活力及增强SOD活力效应比TSG或TEN单用组效应更好(P<0.01);(3)PC12细胞的分化率高低为:TSG-TEN组>多奈哌齐组>TSG组>TEN组(P<0.01,P<0.05);TSG组、TEN组、多奈哌齐组均低于TSG-TEN配伍组(P<0.01,P<0.05)。结论TSG和TEN配伍对Aβ25-35诱导的PC12细胞损伤具有显著的协同保护作用,其作用机制可能与改善胆碱能系统、抗氧化应激、降低突触可塑性损伤有关,且TSG优于TEN。Objective To investigate the effects of tetrahydroxy stilbene glycoside(TSG)and tenuigenin(TEN)on the injury of PC12 cells induced by Aβ25-35.Methods PC12 cells were induced by Aβ25-35 to establish a PC12 cell model of Alzheimer’s disease(AD).After modeling,PC12 cells were randomly divided into a blank group,a model group,a TSG(200 mmol/L)group,a TEN group(100 mg/L),a TSG-TEN compatibility group(TSG 200 mmol/L+TEN 100 mg/L)and a donepezil group(10 umol/L).MTT colorimetry was used to detect cell viability in each group;The lactate dehydrogenase(LDH)and malondialdehyde(MDA)content,acetylcholinesterase(AchE)and superoxide dismutase(SOD)activity in cell supernatant of each group were measured.The cell differentiation rate of each group was caculated.Results(1)The cell survival rate was TSG-TEN group﹥TSG group﹥donepezil group﹥TEN group(P<0.01).Compared with the TSG-TEN combination group,the TSG group,the TEN group and the donepezil group were lower than the TSG-TEN compatibility group(P<0.01,P<0.05).(2)Compared with the model group,TSG group,TEN group and TSG-TEN combination group could decrease LDH and MDA content,inhibit the activity of AchE,and increase SOD activity(P<0.05),and the effect of TSG+TEN compatibility group on reducing the content of LDH and MDA,inhibiting the activity of AchE,and increasing SOD activity was better than that of TSG or TEN alone group(P<0.01).(3)The differentiation of PC12 cells was:TSG-TEN group﹥donepezil group﹥TSG group﹥TEN group(P<0.01).Compared with the TSG-TEN combination group,the TSG group,the TEN group and the donepezil group were lower than the TSG-TEN compatibility group(P<0.01).Conclusion The combination of TSG and TEN has a synergistic protective effect on PC12 cell injury induced by Aβ25-35.The mechanism may be related to the improvement of cholinergic system,anti-oxidative stress and reducing synaptic plasticity damage,and TSG is superior to TEN.

关 键 词：阿尔茨海默病 远志总皂苷 二苯乙烯苷 突触可塑性 

分 类 号：R285.5[医药卫生—中药学]