绵阳地区山羊粪便中捻转血矛线虫的分子鉴定  

Molecular identification of Haemonchus contortus in faeces of goats from Mianyang area

作　　者：蒲善秋 简克灵 李波 谢跃[1] 何冉 杨光友[1] 古小彬[1] Pu Shanqiu;Jian Keling;Li Bo(College of Veterinary Medicine,Sichuan Agricultural University,Sichuan Chengdu 611130;Animal Disease Prevention and Control Center of Mianyang City,Sichuan Mianyang 621000,China)

机构地区：[1]四川农业大学动物医学院,四川成都611130 [2]四川省绵阳市动物疫病预防控制中心,四川绵阳621000

出　　处：《四川畜牧兽医》2020年第1期25-28,共4页Sichuan Animal & Veterinary Sciences

基　　金：四川农业大学学科“双支计划”(SC-03573070)

摘　　要：为鉴定绵阳地区山羊粪便中疑似血矛属线虫虫卵的虫种,本研究提取绵阳地区三个县山羊粪便中疑似血矛属线虫的虫卵DNA 60份,采用PCR技术扩增其核糖体第二内转录间隔区(rDNA ITS-2)基因序列,并与GenBank上公布的捻转血矛线虫同源序列进行比较分析。结果显示:60份疑似血矛属线虫的虫卵DNA样品均得到191bp的ITS-2片段;60条ITS-2序列共形成15条不同的序列,含有17个单变异位点,与GenBank中多株捻转血矛线虫的同源性达95.8%~100%;ML系统发育树显示,本研究得到的15种类型ITS-2序列与已报道的捻转血矛线虫(Haemonchus contortus)聚类形成一个分支。综上可得,60份来自山羊的血矛属线虫均为捻转血矛线虫。To identify the suspected Haemonchus sp.egg specimens in faeces of goats from three Counties in Mianyang area,60 egg specimens were used for DNA extraction,and the internal transcribed spacers(rDNA ITS-2)were amplified and sequenced,then compared with other homologous sequences of H.contortus available from GenBank.The results showed that ITS-2 sequences were successfully amplified from 60 egg specimens,and the length of ITS-2 sequence alignments covered 191 bp.The 60 sequences were classified into 15 different sequences,containing 17 variable sites.The similarity of ITS-2 sequences varied from 95.8 to 100.0%among our sequences and other H.contortus sequences from GenBank.The to⁃pologies in ML phylogenetic tree showed that the 15 different ITS-2 sequences obtained in this study and other reported H.contortus clustered in the same clade.These results indicated that all 60 specimens from goats in Mianyang area were Haemonchus contortus.

关 键 词：捻转血矛线虫 山羊 分子鉴定 ITS-2 基因多态性 

分 类 号：S852.731[农业科学—基础兽医学]