中国明对虾急性肝胰腺坏死病(AHPND)和池塘水体中弧菌的检测分析  

The detection and analysis of acute hepatopancreatic necrosis disease (AHPND) and Vibrio in water or in Fenneropenaeus chinensis

作　　者：陈晓玲 邱亮[2] 魏海英 王君霞 孙成峰 许传堂 CHEN Xiaoling;QIU Liang;WEI Haiying;WANG Junxia;SUN Chengfeng;XU Chuantang(Rizhao Ocean Fisheries Institute,Rizhao 276826,China;Laboratory for Marine Fisheries Science and Food Production Processes,National Laboratory for Marine Science and Technology(Qingdao),Key Laboratory of Maricultural Organism Disease Control,Ministry of Agriculture and Rural Affairs,Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China)

机构地区：[1]日照市海洋与渔业研究所,山东日照276826 [2]中国水产科学研究院黄海水产研究所,青岛海洋科学与技术试点国家实验室海洋渔业功能实验室,农业农村部海水养殖病害防治重点实验室,青岛市海水养殖流行病学与生物安保重点实验室,山东青岛266071

出　　处：《渔业研究》2020年第1期22-28,共7页Journal of Fisheries Research

基　　金：国家虾蟹产业技术体系建设任务书(CARS-48)

摘　　要：近年来,对虾急性肝胰腺坏死病(AHPND)在全球范围内暴发,给对虾养殖业造成了严重的损失。为了进一步掌握中国明对虾AHPND的暴发与池塘水体中弧菌数量的关系,本文采用套式PCR法以及TCBS选择培养基法,分别对2018年4—9月采集的样品进行AHPND致病基因检测和池塘水体中弧菌数量的分析。套式PCR检测结果显示,7、8月AHPND检出率较高,分别达到15.6%、25.0%,其他月份未有阳性样品检出;TCBS选择培养基结果显示,在调水、控料等措施之下,4月至9月期间各虾池的弧菌数量控制在0~8.6×103 cfu·mL-1之内,其中7、8月达到高峰。另外,将本次试验中发现的TCBS黑色弧菌菌落,进行16S rRNA菌落PCR并测序,运用Mega 6软件中的Neighbor-Joining法构建系统进化树,结果显示其与溶藻弧菌(Vibrio alginolyticus)亲缘关系较近。本文针对该病的基本情况,结合近几年国内外的研究进展,提出了一些防控措施,为进一步控制该病害的发生提供了依据。In recent years,acute hepatopancreatic necrosis disease(AHPND)has broken out in all over the world and led to significant economic losses to shrimp farms.To gain further information of the outbreak of AHPND and Vibrios in Fenneropenaeus chinensis ponds,nPCR method and TCBS selective medium culture were used to detect samples collected from April to September 2018,respectively.The nPCR products revealed that the positive detection rate was higher in July and August,reached 15.6%and 25.0%respectively,whereas no positive samples were detected in other months.The TCBS selected medium results showed that Vibrios were controlled within 0~8.6×103 cfu·mL 1 between April and September,under the measures of water transfer and material control,and peaked in July and August.Furthermore,the black colony found in this experiment was detected by 16S rRNA colony PCR,while the neighbor-joining method in Mega 6 was used to construct phylogenetic tree.In this paper,based on the basic information of this disease and combined with the research progress in recent years,we proposed a series of control measures,which provided a basis for further controlling the occurrence of the disease.

关 键 词：中国明对虾 急性肝胰腺坏死病 弧菌 TCBS 菌落PCR 防控措施 

分 类 号：S945.1[农业科学—水产养殖]