蜡样芽孢杆菌PAS38对肉仔鸡生长性能及免疫功能的影响  

Effects of Bacillus cereus PAS38 on Growth Performance and Immune Function of Broilers

作　　者：张娇[1] 王振华[2] 潘康成[1] 倪学勤[1] 李佳骏 李建臻 谷笑笑[1] ZHANG Jiao;WANG Zhenhua;PAN Kangcheng;NI Xueqin;LI Jiajun;LI Jianzhen;GU Xiaoxiao(College of Veterinary Medicine, Sichuan Agricultural University, Animal Microecology Research Center, Chengdu 611130, China;Chengdu Vocational College of Agricultural Science and Technology, Chengdu 611130, China)

机构地区：[1]四川农业大学动物医学院,动物微生态研究中心,四川成都611130 [2]成都农业科技职业学院,四川成都611130

出　　处：《华北农学报》2019年第S01期340-345,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金资助项目(31472130);四川省高等学校科技创新团队资助项目(KM406183.1);四川农业大学学科双支计划项目(03571146)

摘　　要：为了研究蜡样芽孢杆菌PAS38制剂对肉鸡生长性能和免疫功能的影响,选用80只7日龄爱拔益加(AA)肉鸡,随机分成2组,每组4个重复,每个重复10羽,对照组饲喂基础日粮,试验组在基础日粮中添加0.05%的蜡样芽孢杆菌PAS38制剂,试验期35 d。结果显示:试验组鸡体质量及净增质量均比对照组高,但仅在28 d时差异显著(P<0.05),空肠和回肠淀粉酶和蛋白酶活性均显著或极显著高于对照组(P<0.05或P<0.01);试验组的胸腺和脾脏指数在28,42 d时显著或极显著高于对照组(P<0.05或P<0.01),法氏囊指数在42 d时显著高于对照组(P<0.05);试验组血清免疫球蛋白含量均高于显著或极显著高于对照组(P<0.05或P<0.01),42 d时试验组血清中IL-2和IFN-γ含量高于对照组(P>0.05),IL-4含量低于对照组(P>0.05);试验组肉鸡的胸腺和法氏囊组织中IL-2、IFN-γ、GBP1、IRF1的mRNA表达量均有不同程度地高于对照组,IL-10 mRNA转录水平低于对照组;IL-4 mRNA转录水平在胸腺组织中显著高于对照组(P<0.05),而在法氏囊组织中则显著(P<0.05)或极显著(P<0.01)低于对照组。结果表明,蜡样芽孢杆菌PAS38具有促进肉鸡的生长性能和增强肠道消化酶活性,促进免疫器官的发育,并且通过调节免疫相关基因mRNA的表达来提高机体的非特异性免疫和细胞免疫。The paper was designed to investigate the effects of Bacillus cereus PAS38 on growth performance and immune function of broilers.A total of 807-day-old Arbor Acres(AA)broilers were randomly divided into 2 groups with 4 replicates in each group and 10 broilers in per replicate.The control group was fed the basal diet,and the treatment group was fed the basal diet supplemented with 0.05%the additive of Bacillus cereus PAS38.The experiment was conducted for 35 d.The results showed:The body weight and weight gain of broilers in the treatment group were higher than those of the control group,but the difference was significant at 28 d(P<0.05).Amylase and protease activities in the ileum and jejunum of the treatment group were higher than those of the control group(P<0.05 or P<0.01).The index of thymus and bursa fabricii in the treatment group were higher than those of the control group at 28 d and 42 d(P<0.05 or P<0.01),and the bursa fabricii index was significant at 42 d(P<0.05).The serum immunoglobulin content of the treatment group was higher than that of the control group(P<0.05 or P<0.01).The serum IL-2 and IFN-γlevels of the treatment group were higher than those of the control group(P>0.05)and IL-4 content was lower than that of the control group at 42 d(P>0.05).The mRNA expression levels of IL-2,IFN-γ,GBP1 and IRF1 in the thymus and the bursa fabricii of the treatment group were higher than those of the control group,and the IL-10 mRNA transcription level was lower than that of the control group;IL-4 mRNA transcription level of the treatment group was significantly higher than that of the control group in the thymus(P<0.05),but it was significantly lower(P<0.05)and extremely significant(P<0.01)in the bursa fabricii.It is concluded that Bacillus cereus PAS38 could promote the growth performance of broilers,increase the activities of intestinal digestive enzymes,stimulate the development of immune organs,and improve the non-specific immunity and cellular immunity by regulating the expression of mRNA of immune-r

关 键 词：肉鸡 蜡样芽孢杆菌 生长性能 消化酶 免疫功能 

分 类 号：S432.4[农业科学—植物病理学]