芎芍胶囊含药血清对RAW264.7源性泡沫细胞胆固醇流出的影响  

Effect of Xiongshao Capsule Containing Serum on Cholesterol Efflux in Foam Cells from RAW264.7 Cells

作　　者：梅俊 周庆兵[2] 徐凤芹[2] MEI Jun;ZHOU Qing-bing;XU Feng-qin(Graduate School,China Academy of Chinese Medical Sciences,Beijing 100700;Institute of Gerontology,Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091)

机构地区：[1]中国中医科学院研究生院,北京100700 [2]中国中医科学院西苑医院老年医学研究所,北京100091

出　　处：《中国中西医结合杂志》2020年第1期65-69,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81473529);中国中医科学院自主课题资助项目(No.220808043)

摘　　要：目的观察芎芍胶囊大鼠含药血清中脂质对RAW264.7巨噬细胞源性泡沫细胞胆固醇流出的影响。方法采用80 mg/L氧化低密度脂蛋白(ox-LDL)诱导巨噬细胞24 h,建立RAW264.7源性泡沫细胞模型,添加大鼠含药血清,将细胞分为对照组(正常巨噬细胞)、模型组(80 mg/L ox-LDL)、正常血清组(10%正常大鼠血清)及正常血清对照组(80 mg/L ox-LDL+10%正常大鼠血清)、辛伐他汀组(80 mg/L ox-LDL+10%辛伐他汀血清)、芎芍胶囊血清组(80 mg/L ox-LDL+10%芎芍胶囊血清)。通过油红O染色观察细胞内胆固醇分布;以胆固醇检测试剂盒检测正常血清组、辛伐他汀血清组、芎芍胶囊血清组血清总胆固醇(TC)及游离胆固醇(FC)含量,检测各组细胞内TC及FC含量。结果模型组细胞形态及染色结果符合泡沫细胞标准;正常血清组、正常血清对照组、辛伐他汀血清组、芎芍胶囊血清组细胞内均含有大量脂滴,且各组细胞内脂质着色无明显差异。与正常血清组比较,辛伐他汀血清、芎芍胶囊血清组TC差异均无统计学意义(P>0.05),辛伐他汀血清组FC含量升高(P<0.01),芎芍胶囊血清组FC含量降低(P<0.05)。与对照组比较,模型组、正常血清组、正常血清对照组、辛伐他汀血清组细胞内TC、FC含量均增加(P<0.01),芎芍胶囊血清组细胞内TC含量无明显增加(P=0.09),FC含量明显增加(P<0.05)。与模型组比较,芎芍胶囊血清组细胞内TC含量有所降低(P=0.034),FC含量浓度差异无统计学意义(P=0.478);与正常血清对照组比较,芎芍胶囊血清组TC含量降低(P=0.026),FC含量差异无统计学意义(P=0.354)。结论大鼠源性血清本身含有大量脂质,可造成RAW264.7细胞出现胆固醇蓄积,甚至泡沫化,掩盖了芎芍胶囊可能促进泡沫细胞胆固醇流出的作用,不适用于探索RAW264.7源性泡沫细胞胆固醇流出的药物研究。Objective To observe the effect of lipids in Xiongshao Capsule-containing serum(XCS)derived from rats in the research on cholesterol efflux in foam cells from RAW264.7 macrophages.Methods RAW264.7 macrophages were treated with 80 mg/L oxidized low-density lipoprotein(ox-LDL)for 24 h in order to obtain macrophage foam cells.The foam cells were intervened with drug-containing serum derived from rats.The macrophages were assigned to control group(normal macrophages),model group(80 mg/L ox-LDL),normal serum group[10%normal rat serum(NRS)],normal serum control group(80 mg/L ox-LDL and 10%NRS),simvastatin containing serum(SS)group(80 mg/L ox-LDL and 10%SS),XCS group(80 mg/L ox-LDL and 10%XCS).The distribution of cholesterol in cells were evaluated by Oil red O staining.The contents of total cholesterol(TC)and free cholesterol(FC)were assessed using commercial kits.Results The cell morphology and staining results of the model group met the foam cell standard.A large number of lipid droplets were found in all groups except control group,and there was no significant difference in lipid staining between the groups.Compared with the NS group,there was no statistical difference in TC of SS and XCS group(P>0.05),while more FC existed in SS group(P <0. 01) ,yet less in XCS group (P <0. 05) . Compared with the control group,the cellular contents of TC andFC increased in the model group,NS group,NS control group and SS group( AllP <0. 01) ,there was no significant increasein cellular TC of XCS group(P =0. 09) ,but FC of XCS group was markedly increased(P <0. 05) . Compared with the modelgroup,the contents of cellular TC significantly decreased in XCS group(P =0. 034) ,but there was no statistical differencein FC of XCS group (P =0. 478) . Compared with NS control group,the contents of cellular TC significantly decreased (P =0. 026) ,but no obviously difference in FC of XCS group (P =0. 354) . Conclusion XCS derived from rats contains a largenumber of lipids which can cause cholesterol accumulation,even froth in RAW264. 7 cells,a

关 键 词：芎芍胶囊 含药血清 泡沫细胞 胆固醇流出 

分 类 号：R285.5[医药卫生—中药学]