慈菇消脂丸含药血清对非酒精性脂肪肝细胞模型Caspase-8、FasL、p-c-Jun表达的影响  

Effect of Cigu Xiaozhi Pill Containing Serum on the Expression of Caspase-8, FasL, and p-c-Jun in Non-alcoholic Fatty Liver Cell Model

作　　者：马燕花 师霞 武燕 何华 余臣祖 MA Yan-hua;SHI Xia;WU Yan;HE Hua;YU Chen-zu(Clinical Medical School,Gansu University of Chinese Medicine,Lanzhou 730000)

机构地区：[1]甘肃中医药大学临床医学院

出　　处：《中国中西医结合杂志》2020年第1期85-90,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金地区基金项目(No.81560753)

摘　　要：目的探讨慈菇消脂丸含药血清对游离脂肪酸(FFA)混合物诱导的HepG2细胞非酒精性脂肪肝病模型凋亡相关蛋白Caspase-8、FasL、p-c-Jun表达的影响。方法以HepG2细胞为研究对象,实验随机分为正常组(10%胎牛血清),模型组(500μmol/L FFA),中药低、中、高剂量组(500μmol/L FFA+2%、4%、6%含药血清),阳性药组(500μmol/L FFA+20μmol/L SP600125)。实验时间为24 h,实验结束后,用油红O染色镜下观察细胞浆内变化。流式细胞术检测细胞凋亡率。实时荧光定量PCR和Western Blot法检测Caspase-8、FasL、pc-Jun mRNA及蛋白表达水平。结果与正常组比较,模型组细胞凋亡率增加(P<0.01),Caspase-8、FasL、p-c-Jun蛋白及mRNA表达均升高(P<0.05);与模型组比较,各药物干预组及阳性药组细胞凋亡率均下降(P<0.05),除中药低剂量组对FasL蛋白表达及中药中剂量组对Fasl mRNA表达无影响外,其余各组Caspase-8、FasL、p-c-Jun mRNA及Caspase-8、p-c-Jun蛋白表达均下降(P<0.05)。结论慈菇消脂丸可以改善HepG2细胞脂肪变及凋亡,与下调Caspase-8、FasL、p-c-Jun表达相关。Objective To investigate the effects of the Cigu Xiaozhi Pill containing serum on the expression of apoptosis-related proteins Caspase-8,FasL and p-c-Jun in HepG2 cells of non-alcoholic fatty liver disease model induced by free fatty acids(FFA)mixture.Methods HepG2 cells were used as the study object,and were randomly divided into normal group(10%fetal bovine serum),model group(500μmol/L FFA),Cigu Xiaozhi Pill low,middle,high dose group(500μmol/L FFA+2%,4%,6%drug-containing serum),positive drug group group(500μmol/LFFA+20μmol/L SP600125).The experiment lasted for 24 hours.After the experiment,the changes in cytoplasm were observed under the microscope with oil red O staining.The apoptosis rate was determined by flow cytometry.The mRNA and protein expression levels of Caspase-8,FasL and p-c-Jun were detected by real-time quantitative PCR and Western Blot.Results Compared with the normal group,the apoptosis rate was significantly increased(P<0.01),the expressions of Caspase-8,FasL,p-c-Jun protein and mRNA were significantly increased in the model group(P<0.05).Compared with the model group,the apoptosis rates of cells in each treatment group and positive drug group group were significantly decreased(P<0.05).Except the FasL protein expression in the low-dose Chinese medicine group and FasL mRNA expression in middle-dose Chinese medicine group,the expressions of Caspase-8,FasL,p-c-Jun mRNA,Caspase-8 and p-c-jun protein in the other groups were significantly decreased(P<0.05).Conclusion Cigu Xiaozhi Pill could improve the steatosis and apoptosis of HepG2 cells,which was related to the down-regulation of Caspase-8,FasL and p-c-Jun expression.

关 键 词：HepG2细胞 慈菇消脂丸 非酒精性脂肪肝病 CASPASE-8 FASL p-c-Jun 

分 类 号：R285.5[医药卫生—中药学]