循环雌激素水平对裸鼠颗粒脂肪移植转归的影响  

Effect of circulating estrogen level on the outcome of free fat grafting in nude mice

作　　者：吴舒[1] 朱元正[1] 张静[1] 胡玄 易阳艳[1] WU Shu;ZHU Yuanzheng;ZHANG Jing;HU Xuan;YI Yangyan(Department of Plastic Surgery,the Second Affiliated Hospital of Nanchang University,Nanchang Jiangxi,330006,P.R.China)

机构地区：[1]南昌大学第二附属医院整形美容科

出　　处：《中国修复重建外科杂志》2020年第2期220-225,共6页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金资助项目(81660326);江西省教育厅重点项目(GJJ150057);南昌大学第二附属医院青年课题(2016YNQN12040)~~

摘　　要：目的探讨循环雌激素水平对裸鼠颗粒脂肪移植转归的影响。方法取6~8周龄雌性裸鼠18只(体质量20~25 g),随机分为3组(n=6)。去势组采用卵巢切除术制备去势裸鼠模型,高雌激素组及正常雌激素组仅作相同切口进入腹膜后,不切除卵巢组织。术后高雌激素组每3天灌胃雌二醇(0.2 mg/g)1次,共持续30 d;其他两组同时间点等量PBS灌胃。术后30 d取3组裸鼠尾静脉血经ELISA检测雌二醇浓度后,将接受抽脂术的健康女性捐赠的颗粒脂肪注入裸鼠头皮下(0.3 mL/只)。脂肪移植后8周取材大体观察、称重后,制备切片行HE染色、CD31-围脂滴蛋白荧光染色、解耦联蛋白1(uncoupling protein 1,UCP1)免疫组织化学染色和雌激素受体α免疫荧光染色,测量脂肪细胞直径及脂肪组织血管密度;实时荧光定量PCR检测UCP1和雌激素受体αmRNA表达。结果实验期间裸鼠无死亡。ELISA检测显示与正常雌激素组相比,去势组雌二醇浓度明显降低,高雌激素组明显升高,差异均有统计学意义(P<0.05)。脂肪移植8周后,移植物体积从大到小依次为去势组、正常雌激素组、高雌激素组,其中去势组移植物湿重显著高于高雌激素组(P<0.05)。组织学染色显示,与正常雌激素组相比,去势组脂肪细胞增大且围脂滴蛋白表达较弱、血管密度减小,几乎不表达脂肪标记物UCP1,雌激素受体α阳性细胞减少;而高雌激素组上述观察结果与去势组相反;脂肪细胞直径、血管密度及雌激素受体α阳性细胞组间比较,差异均有统计学意义(P<0.05)。实时荧光定量PCR检测显示,与正常雌激素组相比,高雌激素组移植物UCP1和雌激素受体αmRNA相对表达量升高,而去势组均降低;组间比较差异均有统计学意义(P<0.05)。结论体内循环雌激素水平对脂肪移植的转归有显著影响。低雌激素水平导致移植物脂肪细胞肥大,高雌激素水平导致脂肪移植物中生成大量米色脂肪并伴随Objective To investigate the effect of circulating estrogen level on the outcome of free fat grafting in nude mice.Methods Eighteen female nude mice aged 6-8 weeks(weighing,20-25 g)were randomly divided into 3 groups(n=6).The nude mice in the ovariectomized group were treated with ovariectomy.The nude mice in the high estrogen group and the normal estrogen group only made the same incision to enter the peritoneum without ovariectomy.The nude mice in the high estrogen group were given the estradiol(0.2 mg/g)every 3 days for 30 days.The other two groups were given the same amount of PBS every 3 days.At 30 days after operation,the tail vein blood of nude mice in 3 groups were detected by estradiol ELISA kit,and the free fat(0.3 mL)donated by the females was injected into the subscalp of nude mice.After 8 weeks of fat grafting,the samples were taken for gross observation and weighing,and the prepared slices were stained with HE staining,CD31-perilipin fluorescence staining,immunohistochemical staining of uncoupling protein 1(UCP1),and immunofluorescence staining of estrogen receptorα.The diameter of adipocytes and vascular density of adipose tissue were measured.The mRNA expressions of UCP1 and estrogen receptorαwere detected by realtime fluorescence quantitative PCR(qRT-PCR).Results All nude mice survived during experiment.ELISA test showed that the concentration of estradiol significantly decreased in the ovariectomized group and increased in the high estrogen group compared with the normal estrogen group(P<0.05).At 8 weeks after fat grafting,the graft volume from large to small was ovariectomized group,normal estrogen group,and high estrogen group.There was significant difference in wet weight between the ovariectomized group and high estrogen group(P<0.05).Section staining showed that compared with the normal estrogen group,the adipocytes in the ovariectomized group were larger,the expression of peri-lipoprotein was weaker,the vascular density decreased,and the expressions of UCP1 was negative,and the estrogen

关 键 词：雌激素 脂肪移植 血管新生 雌激素受体Α 裸鼠 

分 类 号：R622[医药卫生—整形外科]