电感耦合等离子体-质谱法测定人尿中21种微量元素  

Determination of 21 trace elements in human urine by inductively coupled plasma-mass spectrometry

作　　者：王丽 朱军 吴倩[2] 曹淑媛 李磊[2] 程洁 WANG Li;ZHU Jun;WU Qian;CAO Shu-yuan;LI Lei;CHENG Jie(Department of Core Facilities,School of Public Health,Nanjing Medical University,Nanjing,Jiangsu 211166,China)

机构地区：[1]南京医科大学公共卫生学院科研共享平台,江苏南京211166 [2]南京医科大学公共卫生学院卫生检验学系,江苏南京211166

出　　处：《现代预防医学》2020年第3期484-487,共4页Modern Preventive Medicine

基　　金：江苏高校品牌专业建设工程资助项目(PPZY2015A067);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：目的建立人尿中21种微量元素同时分析的电感耦合等离子体-质谱测定方法。方法尿液经2%硝酸稀释10倍后,采用电感耦合等离子体(ICP)-四级杆质谱法(MS)检测。结果采用内标法定量,各元素内标回归方程的决定系数均大于0.99,加标回收率在63.6%-101.5%之间。所测元素的相对标准偏差小于15%。各元素检出限在0.001μg/L-0.880μg/L之间。尿样内标元素45Sc的信号漂移在40%以内;74Ge,89Y和209Bi的信号漂移在30%以内;115In的信号漂移在20%以内。结论ICP-MS方法简便、快速、灵敏、稳定,适合批量人尿中21种元素的分析。Objective To establish a method for simultaneous analysis of 21 trace elements in human urine by inductively coupled plasma-mass spectrometry.Methods The urine was diluted with 2%nitric acid for 10 times,and detected by inductively coupled plasma-mass spectrometry(ICP-MS).Results Using the internal standard method,the determination coefficients of each element were all greater than 0.99,and the recoveries were between 63.6%~101.5%.The relative standard deviations(RSDs)were less than 15%.The detection limits were between 0.001μg/L and 0.880μg/L The signal drift of 45Sc in the urine sample was within 40%and the signal drift of 74Ge,89Y and 209Bi was within 30%.The signal drift of 115In was within 20%.Conclusion This method is simple,quick,sensitive and stable.Hence,it is suitable for the analysis of 21 trace elements in batch human urine.

关 键 词：尿液 电感耦合-等离子体质谱法 微量元素 

分 类 号：R115[医药卫生—公共卫生与预防医学]