广西南宁市部分腹泻标本诺如病毒检测结果分析  

Results of norovirus detection of some diarrhea specimens in Nanning City, Guangxi Province

作　　者：农皓[1] 刘昊晖[1] 杨丞[1] 尹刘江[1] 詹鑫婕[1] 秦剑秋[1] NONG Hao;LIU Hao-hui;YANG Cheng;YIN Liu-jiang;ZHAN Xin-jie;QIN Jian-qiu(Nanning Center for Disease Control and Prevention,Nanning,Guangxi 530023,China)

机构地区：[1]南宁市疾病预防控制中心

出　　处：《现代预防医学》2020年第3期488-491,共4页Modern Preventive Medicine

基　　金：广西壮族自治区卫生和计划生育委员会自筹经费科研基金(No.Z20170721);2017年南宁市人才小高地专项资金资助项目(No.2017040)

摘　　要：目的了解南宁市部分腹泻患者中诺如病毒感染情况,分析南宁市诺如病毒流行特征。方法收集2015年5月-2019年3月南宁市部分腹泻患者粪便或肛拭子标本1199份,采用实时荧光PCR进行诺如病毒GⅠ型和G Ⅱ型检测,Excel2007整理信息数据.SPSS21.0进行统计学分析。计数资料采用卡方检验,并进行描述性统计分析。结果2015年5月-2019年3月,南宁市部分腹泻人群中诺如病毒总阳性率为19.85%,GⅠ型占9.24%,G Ⅱ型占88.24%,GⅠ型和GⅡ型混合感染占2.52%o 3月和11月阳性率高,武鸣区阳性率最高33.18%;各年龄段、性别、标本类型阳性率差异无统计学意义,各年龄段GⅠ型阳性率差异有统计学意义(F=12.374,P=0.015)。31岁~组阳性率最高。结论南宁市GⅡ型的感染比GⅠ型更加广泛,流行时期是早春和秋季。监测范围应覆盖全市,规范样品采集数量和质量,对阳性率高的年龄组进行重点釆集。开展部分腹泻人群诺如病毒基因测序,为我市诺如病毒预防和控制提供更加科学的数据支持。Objective To understand the status of Norovirus infection in some patients with diarrhea in Nanning,and to analyze the epidemiological characteristics of Norovirus in Nanning.Methods A total of 1199 specimens of feces or anal swabs from patients with diarrhea in Nanning from May 2015 to March 2019 were collected.Real-time fluorescent PCR was used to detect Norovirus GⅠ and GIL Excel 2007 was used to collate the data,SPSS 21.0 was used to carry out the statistical analysis,Chisquare test was used for counting data,and P<0.05 was considered statistically significant.Descriptive statistical analysis was performed.Results From May 2015 to March 2019,the total positive rate of Norovirus in some diarrhea population in Nanning was 19.85%,GⅠ type was 9.24%,and GⅡ type was 8&24%.GⅠ type and GⅡ type mixed infection accounted for 2.52%.The positive rates were high in March and November.The positive rate of Wuming District was the highest,33.18%.There was no significant difference in the positive rate of all age groups,gender and specimen types.There was significant difference in the positive rate of type G I among different age groups(2=12.374,P=0.015).The highest positive rate was found in 31-year-old group.Conclusion The infection of GⅡ type in Nanning is more extensive than that of GⅠ.The epidemic seasons are early spring and autumn.The monitoring scope should cover the whole city,and the quantity and quality of sample collection should be standardized,focusing on the age group with high positive rate.NoV gene sequencing in some diarrhea population should be carried out to provide more scientific data support for NoV prevention and control in Nanning.

关 键 词：诺如病毒 腹泻 基因型 流行特征 

分 类 号：R181.3[医药卫生—流行病学] R512.5[医药卫生—公共卫生与预防医学]