荷花B类MADS-box基因家族NnDEF基因的克隆及表达分析  

Cloning and expression analysis of the putative NnDEF gene from B-class MADS-box family in lotus

作　　者：丁献华[1] 陈伟[2] 张直峰[2] DING Xianhua;CHEN Wei;ZHANG Zhifeng(Department of Economics and Management,Linfen Vocational and Technical College,Linfen 041000,China;School of Life Science,Shanxi Normal University,Linfen 041000,China)

机构地区：[1]临汾职业技术学院经济管理系,临汾041000 [2]山西师范大学生命科学学院,临汾041000

出　　处：《上海农业学报》2020年第1期1-7,共7页Acta Agriculturae Shanghai

基　　金：国家自然科学基金(31701949);山西省自然科学基金(201601D021098)。

摘　　要：以荷花品种‘重瓣一丈青’(Nelumbo nucifera'chongban yizhangqing')为试材,采用同源克隆方法获得2个AP3/DEF同源基因NnDEF1和NnDEF2。NnDEF1基因CDS序列全长678 bp,编码225个氨基酸;NnDEF2基因CDS序列全长693 bp,编码230个氨基酸;2个基因的C-末端序列均包含典型的PI衍生基序和PaleoAP3基序。序列比对和系统进化分析表明,NnDEF1和NnDEF2基因属于B功能基因家族AP3/DEF亚家族,2个基因序列比对其同源性远高于其它物种同源基因,表明荷花进化中可能存在基因加倍事件。表达模式分析显示,NnDEF1和NnDEF2基因表达具有品种间相似性和个体内差异性,NnDEF1倾向于在花瓣和雄蕊组织中表达,尤其内侧花瓣表达量最高;而NnDEF2则表达更广泛,在茎、叶、萼片、花瓣组织中均有表达,在外轮花瓣组织中表达量最高。基因表达差异表明,NnDEF1基因可能在荷花瓣化现象中发挥作用。上述研究结果可为探讨荷花花发育及瓣化现象分子机制的研究奠定基础。Two AP3/DEF-homologous genes NnDEFl and NnDEF2 were cloned by homologous-cloning method from Nelumbo nucifera‘chongban Yizhangqing’.The CDS sequences of NnDEFl and NnDEF2 genes are 678 bp and 693 bp in length,encoding 225 and 230 amino acids,respectively.The C-terminal sequences of the 2 proteins contain typical Pi-derived motif and PaleoAP3 motif.Sequence alignment and phylogenetic analysis results showed that NnDEFl and NnDEF2 genes belonged to AP3/DEF subfamily of B-functional gene family,and their homology was much higher than that of other species,suggesting that there might be genes doubling events in lotus evolution.Expression pattern analysis showed that NnDEFl and NnDEF2 genes had similarities among cultivars and differences within individuals.NnDEFl tended to express in petals and stamens,especially in medial petals,while NnDEF2 was more widely expressed in stem,leaf,sepal and petal tissues,especially in outer petal tissues that had the highest expression level.Gene expression difference indicated that the NnDEFl gene might play a role in the petalody phenomenon.These above results could lay a foundation for studying the molecular mechanism of floral development and petalody phenomenon in lotus.

关 键 词：荷花 基因 MADS-BOX 表达分析 瓣化 

分 类 号：S682.63[农业科学—观赏园艺] Q943.2[农业科学—园艺学]