紫地榆不同极性部位抗炎活性与HPLC图谱相关性研究  

Correlation between Anti-inflammatory Activity and HPLC Spectrum of Different Polar Parts of Geranium strictipes

作　　者：吕兴 卢建升 耿树琼 陈俊雅 耿玲 蓝海 周萍 Lv Xing;Lu Jiansheng;Geng Shuqiong;Chen Junya;Geng Ling;Lan Hai;Zhou Ping(College of Pharmacy and Chemistry,Dali University,Dali,Yunnnan 671000,China;Pre-clinical College,Dali University,Dali,Yunnan 671000,China)

机构地区：[1]大理大学药学与化学学院,云南大理671000 [2]大理大学基础医学院,云南大理671000

出　　处：《大理大学学报》2020年第2期31-37,共7页Journal of Dali University

基　　金：国家自然科学基金资助项目(30860365);国家级创新训练基金资助项目(KYXL2018-08);云南省教育厅科学研究基金资助项目(2019J0760;2019Y0261)

摘　　要：目的:研究紫地榆乙醇提取物及其不同极性部位高效液相色谱(HPLC)图谱与其体内抗炎活性的谱效相关性,探讨紫地榆发挥抗炎活性的物质基础。方法:建立紫地榆不同部位的HPLC图谱,采用二甲苯致小鼠耳廓肿胀法测定紫地榆不同部位的抗炎活性,采用双变量相关分析法和偏最小二乘回归分析法研究其不同部位HPLC特征色谱峰与其抗炎药效之间的谱效关系。结果:紫地榆不同部位均有抗炎作用,其中乙酸乙酯部位作用最佳。HPLC图谱所标定的27个特征色谱峰中,有13个与抗炎率呈显著相关,分别为1(没食子酸)、2(没食子酸甲酯)、3(儿茶素)、8、11、13、15、17、19(五没食子酰葡萄糖)、20、21、25、27号峰。结论:紫地榆抗炎活性作用是其多个成分共同作用的结果。Objective:To study the correlation among the spectra-effect relationship of HPLC spectrums of ethanol extracts and their polar parts of Geranium strictipes and their anti-inflammatory activities in vivo,and explore the material basis of the anti-inflammatory activity of Geranium strictipes.Methods:The HPLC spectrums of different parts of Geranium strictipes were established.The antiinflammatory activities of different parts of Geranium strictipes were determined by xylene-induced mouse ear swelling method.The spectral-effect correlation between HPLC characteristic peaks of different parts and their anti-inflammatory effects was analyzed by bivariate correlation analysis and partial least squares regression analysis.Results:Different parts of Geranium strictipes all exhibited anti-inflammatory effects in which the ethyl acetate part worked best.The 27 characteristic chromatographic peaks in the HPLC spectrums were selected,and 13 peaks were significantly correlated with the anti-inflammatory rate,the peaks ranked as No.1(gallic acid),2(methyl gallate),3(catechin),8,11,13,15,17,19(pentagalloylglucose),20,21,25 and 27.Conclusion:The antiinflammatory activity of Geranium strictipes is the result of the coaction of multiple components.

关 键 词：紫地榆 抗炎活性 HPLC图谱 谱效关系 

分 类 号：R285.5[医药卫生—中药学]