膝骨性关节炎者TLR-4、IL-1β的表达变化及临床意义  

Expression changes and clinical significance of TLR-4 and IL-1β in serum and knee joint fluid of patients with knee osteoarthritis

作　　者：毛洪刚[1] 朱艳丽 刘岩[1] 刘国栋[1] 张飞[1] MAO Honggang;ZHU Yanli;LIU Yan;LIU Guodong;ZHANG Fei(Department of Orthopedics,the Third Affiliated Hospital of Inner Mongolia Medical University(Inner Mongolia Baogang Hospital),Inner Mongolia Autonomous Region,Baotou 014010,China;Department of Anesthesiology,the Second Affiliated Hospital of Baotou Medical College,Inner Mongolia University of Science and Technology,Inner Mongolia Autonomous Region,Baotou 014030,China)

机构地区：[1]内蒙古医科大学第三附属医院(内蒙古包钢医院)骨科,内蒙古包头014010 [2]内蒙古科技大学包头医学院第二附属医院麻醉科,内蒙古包头014030

出　　处：《中国医药导报》2020年第3期82-85,共4页China Medical Herald

基　　金：中国金属学会冶金安全与健康分会健康卫生科研项目(jkws201835)

摘　　要：目的探讨膝骨性关节炎(KOA)患者血清、膝关节液中Toll样受体-4(TLR-4)、白细胞介素-1β(IL-1β)的表达变化及临床意义。方法选择2017年6月~2018年12月内蒙古医科大学第三附属医院(以下简称“我院”)骨科门诊收治的KOA患者220例作为观察组,根据KOA的严重程度,将其分为轻度亚组(n=98)、中度亚组(n=80)和重度亚组(n=42),另外选取我院健康中心体检志愿者60名作为对照组。酶联免疫吸附试验检测血清和关节液中TLR-4和IL-1β水平,分析TLR-4和IL-1β水平与KOA严重程度及两者之间的相关性。结果观察组血清及关节液中TLR-4和IL-1β水平均明显高于对照组,差异均有统计学意义(均P<0.05)。重度亚组血清及关节液中TLR-4和IL-1β水平高于轻度亚组和中度亚组,中度亚组血清及关节液TLR-4和IL-1β水平高于轻度亚组,差异均有统计学意义(均P<0.05)。血清和关节液TLR-4和IL-1β水平与KOA的严重程度呈正相关(r=0.706、0.729、0.741、0.715,均P<0.05);血清TLR-4水平与IL-1β水平呈正相关(r=0.720,P<0.05);关节液TLR-4水平与IL-1β水平呈正相关(r=0.736,P<0.05)。结论KOA患者血清和关节液中TLR-4和IL-1β水平异常升高,且与KOA的严重程度联系密切,可以用于临床上KOA的预测、诊断及治疗。Objective To investigate the expression changes and clinical significance of Toll-like receptor-4(TLR-4)and interleukin-1β(IL-1β)in serum and knee joint fluid of patients with knee osteoarthritis(KOA).Methods From June 2017 to December 2018,220 cases with KOA who admitted in Department of Orthopedics,the Third Affiliated Hospital of Inner Mongolia Medical University(“our hospital”for short)were selected as observation group.According to the severity of KOA,they were divided into mild subgroup(n=98),moderate subgroup(n=80)and severe subgroup(n=42).In addition,60 cases of volunteers were selected for physical examination in our hospital of health center medical as control group.The levels of TLR-4 and IL-1β in serum and knee joint fluid were detected by enzyme-linked immunosorbent assay.The correlation between TLR-4,IL-1β levels and KOA severity were analyzed and their correlation were analyzed.Results The levels of TLR-4 and IL-1β in serum and knee joint fluid of observation group were significantly higher than those of control group,and the differences were statistically significant(all P<0.05).The levels of TLR-4 and IL-1β in severe subgroup were significantly increased than those of moderate subgroup and mild subgroup,compared with mild subgroup,the levels of TLR-4 and IL-1β in moderate subgroup were significantly increased,and the differences were statistically significant(all P<0.05).Serum and knee joint fluid TLR-4 and IL-1βlevels were positively correlated with KOA severity(r=0.706,0.729,0.741,0.715,all P<0.05).Serum TLR-4 level was positively correlated with IL-1β level(r=0.720,P<0.05).The level of TLR-4 in knee joint fluid was positively correlated with IL-1 level(r=0.736,P<0.05).Conclusion The levels of TLR-4 and IL-1β in serum and knee joint fluid of KOA patients unusually increase,which is closely related with the severity of KOA.It can be used for clinical prediction,diagnosis and treatment of KOA.

关 键 词：膝骨性关节炎 血清 膝关节液 TOLL样受体-4 白细胞介素-1Β 相关性 

分 类 号：R684.3[医药卫生—骨科学]