Purification and Characterization of a Novel Lipase from Antarctic Krill  

作　　者：CHEN Xin WANG Chunlan XU Jiakun WANG Fang JIANG Yihui CHEN Yixuan and ZHAO Xianyong 

机构地区：[1]College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China [2]Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Sustainable Development of Polar Fishery,Ministry of Agriculture and Rural Affairs of PRC,Qingdao 266071,China [3]Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China [4]Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266071,China [5]College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266100,China

出　　处：《Journal of Ocean University of China》2020年第1期209-215,共7页中国海洋大学学报（英文版）

基　　金：supported by the Marine S&T Fund of Shandong Province for the Pilot National Laboratory for Marine Science and Technology (Qingdao) (Nos. 2018 SDKJ0304-4-2, 2018SDKJ0303-1);the Central Public-Interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences (Nos. 2017HY-XKQ01-01, 2016ZD0902, 2018GH10);the Central Public-Interest Scientific Institution Basal Research Fund, YSFRI, CAFS (No. 20603022018025);the Aoshan S&T Innovation Project from Qingdao National Laboratory for Marine Science and Technology (No. 2015ASKJ02-02-04);the Antarctic Marine Biological Resources Development and Utilization Project from the Ministry of Agriculture and Rural Affairs, People’s Republic of China (2017);the Financial Fund of the Ministry of Agriculture and Rural Affairs, People’s Republic of China (Nos. NFZX2018, FSTICE2019)

摘　　要：Lipase from Antarctic krill,with a molecular weight of 71.27kDa,was purified with ammonium sulfate precipitation and a series of chromatographic separations over ion exchange(DEAE)and gel filtration columns(Sephacryl S-100),resulting in 5.2%recovery with a 22.4-fold purification ratio.The optimal pH and temperature for enzyme activity were 8.0 and 45℃,respectively.Purified lipase had Km and Vmax values of 3.27mmolL−1 and 2.4Umg−1,respectively,using p-nitrophenyl laurate as the substrate.Lipase activity was enhanced by adding Ca2+and Mg2+ions in the concentration ranges of 0–0.5mmolL−1 and 0–0.3mmolL−1,respectively,while the activity was inhibited by a further increase in these ion concentrations.Fe3+and Cu2+ions showed obvious inhibitory effects on enzyme activity,and the inhibition rates were 71.8%and 53.3%when the ion concentrations were 0.5mmolL−1.Lipase from Antarctic krill, with a molecular weight of 71.27 kDa, was purified with ammonium sulfate precipitation and a series of chromatographic separations over ion exchange（DEAE） and gel filtration columns（Sephacryl S-100）, resulting in 5.2% recovery with a 22.4-fold purification ratio. The optimal pH and temperature for enzyme activity were 8.0 and 45℃, respectively. Purified lipase had Km and Vmax values of 3.27 mmol L-1 and 2.4 U mg-1, respectively, using p-nitrophenyl laurate as the substrate. Lipase activity was enhanced by adding Ca2+ and Mg2+ ions in the concentration ranges of 0–0.5 mmol L-1 and 0–0.3 mmol L-1, respectively, while the activity was inhibited by a further increase in these ion concentrations. Fe3+ and Cu2+ ions showed obvious inhibitory effects on enzyme activity, and the inhibition rates were 71.8% and 53.3% when the ion concentrations were 0.5 mmol L-1.

关 键 词：Antarctic krill LIPASE isolation and purification enzymology properties 

分 类 号：S917.4[农业科学—水产科学]