一种呋喃西林酶联免疫检测方法的建立  

Establishment of Enzyme-Linked Immunosorbent Assay for the Determination of Nitrofurazone

作　　者：李泳宁[1] 彭臻菲[1] 黄慧[1] 蔡美虹 林清清 LI Yong-ning;PENG Zhen-fei;HUANG Hui(Fujian Health College,Fuzhou,Fujian 350101)

机构地区：[1]福建卫生职业技术学院

出　　处：《安徽农业科学》2020年第4期193-195,共3页Journal of Anhui Agricultural Sciences

基　　金：福建卫生职业技术学院科技计划项目(2013-1-2)

摘　　要：[目的]建立一种饲料中呋喃西林酶联免疫检测方法。[方法]利用制备的人工抗原和高特异性单克隆抗体,采用间接竞争酶联免疫技术建立呋喃西林检测方法。[结果]在优化条件下,检测抗原浓度为10μg/mL,抗体稀释度为1∶5000。呋喃西林在0.1~10.0 ng/mL具有较好的线性关系(R 2=0.9987),IC 50为1.43 ng/mL。与呋喃唑酮、呋喃它酮、呋喃妥因和呋喃西林代谢物氨基脲的交叉反应率低于0.5%。饲料样品中添加呋喃西林的平均回收率为87.5%~96.0%,变异系数为6.1%~9.7%。[结论]该方法快速、准确、灵敏,可用于饲料中呋喃西林的分析检测。[Objective]To establish an enzyme-linked immunosorbent assay(ELISA)for the determination of nitrofurazone in feed.[Method]The artificial antigen and the high specificity monoclonal antibodies against nitrofurazone were prepared.An indirect competitive ELISA for the determination of nitrofurazone in feed was established.[Result]The optimization of ELISA parameters were that the concentration of antigen was 10μg/mL and the dilution multiples of antibodies was 1∶5000.The good linear range was 0.1-10.0 ng/mL(R 2=0.9987)and IC 50 was 1.43 ng/mL.The cross-reactivity(CR)rate was less than 0.5%with other nitrofurans such as furazolidone,furaltadone,furantoin and semicarbazide.The average recovery from artificially contaminated nitrofurazone of feed was 87.5%-96.0%,with coefficient of variation(CV)was 6.1%-9.7%.[Conclusion]The method developed is rapid,accurate and sensitive and will be helpful for rapid screening for nitrofurazone in feed.

关 键 词：呋喃西林 酶联免疫检测 单克隆抗体 饲料 

分 类 号：R446.6[医药卫生—诊断学]