灰兜巴乙酸乙酯部位的化学成分及α-葡萄糖苷酶抑制活性研究  

Study on the Chemical Constituents and Inhibitory Activity against α-Glucosidase of Ethyl Acetate Extract from Huidouba

作　　者：蔡伟兰 韩慢慢 孙彦敏 李知敏 彭亮 Cai Weilan;Han Manman;Sun Yanmin;Li Zhimin;Peng Liang(Jiangxi Provincial Key Laboratory of Drug Design and Evaluation,School of Pharmacy,Jiangxi Science&Technology Normal University,Nanchang 330013,Jiangxi,China)

机构地区：[1]江西省药物分子设计与评价重点实验室江西科技师范大学药学院

出　　处：《江西科技师范大学学报》2019年第6期60-63,共4页Journal of Jiangxi Science & Technology Normal University

基　　金：江西省青年科学基金（20122BAB215019）;江西科技师范大学研究生创新基金（YC2017-X23）

摘　　要：本文研究了灰兜巴乙酸乙酯部位的化学成分,及各成分对α-葡萄糖苷酶的抑制作用。采用柱层析技术对灰兜巴乙酸乙酯萃取部分进行分离和纯化,利用NMR对分离得到的化合物进行结构鉴定,并用高效液相色谱法开展α-葡萄糖苷酶抑制活性的研究。首次从灰兜巴中分离得到5种化合物,分别为豆甾醇(1)、α-菠菜甾醇(2)、β-谷甾醇(3)、表没食子儿茶素没食子酸酯(4)、咖啡因(5),其中表没食子儿茶素没食子酸酯有较高的α-葡萄糖苷酶抑制作用。灰兜巴中抗糖尿病的活性成分可能来源于周围的环境,而非灰兜巴本身。To study the chemical constituents of ethyl acetate extract from Huidouba,and theirα-glucosidase inhibitory activity.The chemical constituents were separated and purified by column chromatography technique.The structures of these compounds were identified by NMR.Then theα-glucosidase inhibitory activities were tested by HPLC-DAD.Five compounds were isolated and identified from Huidouba for the first time,which were Stigmasterol(1),α-Spinasterol(2),β-Sitosterol(3),Epigallocatechin gallate(EGCG,4),Caffeine(5).EGCG showed obvious inhibitory activity againstα-glucosidase.The anti-diabetes compounds in Huidouba may come from the surrounding environment,but not huidouba itself.

关 键 词：灰兜巴 乙酸乙酯部分 化学成分 Α-葡萄糖苷酶 

分 类 号：R914.1[医药卫生—药物化学]