钛网表面含hBMP-2的复合涂层制备及hBMP-2的释放研究  

hBMP-2 Contained Composite Coatings on Titanium Mesh Surface: Preparation and hBMP-2 Release

作　　者：付亚康 翁杰[2] 刘耀文[3] 张科宏[1] FU Ya-Kang;WENG Jie;LIU Yao-Wen;ZHANG Ke-Hong(Ya’an Polytechnic College,Ya’an 625000,China;Key Laboratory of Advanced Technologies of Materials,Ministry of Education,School of Materials Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China;School of food,Sichuan Agricultural University,Ya’an 625014,China)

机构地区：[1]雅安职业技术学院,雅安625000 [2]西南交通大学材料科学与工程学院,材料先进技术教育部重点实验室,成都610031 [3]四川农业大学食品学院,雅安625014

出　　处：《无机材料学报》2020年第2期173-178,共6页Journal of Inorganic Materials

基　　金：国家自然科学基金(51572228);四川省教育厅自然科学基金(16ZB0546);雅安职业技术学院自然科学基金(2016yzk15)~~

摘　　要：为提高骨接合钛网的骨整合性和生物活性,本研究采用碱热处理法在钛网表面构建出具有多孔结构的钛酸盐纳米纤维,利用电化学沉积技术在钛酸盐纳米纤维表面制备磷酸钙涂层,并采用不同方法将人骨形态发生蛋白(hBMP-2)引入涂层,制备了三种含hBMP-2分子的复合涂层(TmhB、TmHedhB和TmHhBed)。实验对各复合涂层的表面形貌、化学成分、相组成和hBMP-2的含量与释放性能进行了表征。研究发现:各涂层都具有多孔纤维结构,TmHedhB和TmHhBed中的磷酸钙相为羟基磷灰石(HA),呈“串珠”状包裹在钛酸盐纳米纤维表面,“串珠”状HA的引入促进了复合涂层对hBMP-2的吸附。电化学共沉积技术在钛酸盐纳米纤维表面制备的HA/hBMP-2复合涂层中hBMP-2的含量最大,达886 ng/mg,在6~48 h内具有明显的hBMP-2缓释性能。Titanate nanofibers were prepared on titanium mesh by alkali-heat treatment,and calcium phosphate coating was fabricated on the porous titanate nanofibers by electrochemical deposition technology.Then hBMP-2 was introduced into the coating by different methods to improve its osteointegration and bioactivity.Three kinds of composite coatings modified by hBMP-2 were prepared(TmhB,TmHedhB and TmHhBed).Surface morphology,chemical composition,phase composition and hBMP-2 amounts and hBMP-2 release performance of the composite coatings were characterized by SEM,ATR-FTIR,XRD,and hBMP-2 ELISA kit,respectively.Results showed that all of the coatings display porous fiber structure,calcium phosphate phase in TmHedhB and TmHhBed samples was hydroxyapatite(HA),and bead-like HA particles formed on the surface of titanate nanofibers.Protein adsorption experiments showed that introduction of bead-like HA phase increased the hBMP-2 adsorption on the composite coatings,and composite coatings prepared by electrochemical co-deposition technique further enhanced hBMP-2 adsorption up to886 ng/mg,which were supported hBMP-2 sustained release within 6-48 h.

关 键 词：钛 碱热处理 电化学沉积 羟基磷灰石 复合涂层 HBMP-2 

分 类 号：R318[医药卫生—生物医学工程] TQ174[医药卫生—基础医学]