^18F-Flurpiridaz与^13N-NH3·H2O PET/CT心肌灌注显像的动物实验对比研究  

Comparison of ^18F-Flurpiridaz and ^13N-NH3·H2O PET/CT myocardial perfusion imaging in animal experiments

作　　者：汪娇[1] 李帅 刘天文[1] 张杰民[1] 陈越[1] 李剑明[1] Wang Jiao;Li Shuai;Liu Tianwen;Zhang Jiemin;Chen Yue;Li Jianming(Department of Nuclear Medicine,Teda International Cardiovascular Hospital,Tianjin Medical University Clinical Cardiovascular Institute,Tianjin 300457,China)

机构地区：[1]泰达国际心血管病医院核医学科、天津医科大学心血管病临床学院,300457

出　　处：《中华核医学与分子影像杂志》2020年第2期75-81,共7页Chinese Journal of Nuclear Medicine and Molecular Imaging

基　　金：天津市自然科学基金(17JCYBJC28200);天津市滨海新区卫生计生委科技项目(2018BWKZ007)。

摘　　要：目的探讨新型心肌灌注显像(MPI)药物2-叔丁基-4-氯-5-[4-(2-氟-^18F-乙氧基甲基)苯基甲氧基]-3(2H)-哒嗪酮(^18F-Flurpiridaz)在小型猪体内的生物分布及定量分析价值,并与^13N-NH3·H2O进行对比。方法取10只巴马小型猪,分为正常组与心肌梗死组,每组各5只。正常组不行处理,心肌梗死组采用外科开胸结扎冠状动脉的方式建模。2组均先行^13N-NH3·H2O显像,后行^18F-Flurpiridaz显像,2种显像至少间隔40 min,注射剂量均为^185~370 MBq。正常组另行^18F-Flurpiridaz全身显像,观察^18F-Flurpiridaz的生物分布,计算心肌与邻近组织或器官^18F-Flurpiridaz的放射性摄取比值。比较正常组2种显像图像质量及静息心肌血流量(rMBF);比较心肌梗死组2种显像图像质量及静息总评分(SRS)、心肌梗死面积百分比、总灌注缺损(TPD)、左心室射血分数(LVEF)等常用心功能评价参数。采用配对t检验分析数据。结果全身显像示正常组小型猪^18F-Flurpiridaz MPI心肌显影清晰,注射后2 h内始终保持高放射性摄取,左心室心肌与心血池、肺和肝的放射性计数比值高(5.19~12.87、4.17~50.51、2.08~6.92)。2组动物的^18F-Flurpiridaz MPI图像全部(10/10)质量优秀。^18F-Flurpiridaz与^13N-NH3·H2O显像测得的左心室不同区域rMBF(ml·g-1·min-1)差异均无统计学意义(左前降支:0.98±0.06和0.92±0.^13;左回旋支:0.98±0.05和0.88±0.12;右冠状动脉:0.95±0.07和0.88±0.15;左心室:0.96±0.07和0.90±0.^13;t值:-1.70^-0.90,均P>0.05);两者所得的心功能参数差异亦无统计学意义[SRS:10.6±4.1和9.2±4.6;心肌梗死面积百分比:(15.2±9.0)%和(12.6±6.6)%;TPD:(11.6±6.3)%和(9.6±3.9)%;LVEF:(68.6±11.1)%和(71.4±11.3)%;t值:-2.33~2.75,均P>0.05]。结论与^13N-NH3·H2O相比,^18F-Flurpiridaz MPI图像质量好、心功能参数测定准确,具备心肌血流定量的潜力,是一种具有广阔应用前景的正电子MPI药物。Objective To explore the biodistribution and quantitative value of^18F-Flurpiridaz in mini-swine,and compare with^13N-NH3·H2O.Methods Ten Bama mini-swine were divided into normal group and myocardial infarction group(n=5 in each group).Normal group was not treated and myocardial infarction group was modeled by thoracotomy and coronary artery ligation.Both groups were preceded by^13N-NH3·H2O imaging,followed by^18F-Flurpiridaz imaging(time interval>40 min).Injection dosage of 2 tracers was the same(^185-370 MBq).^18F-Flurpiridaz whole-body PET/CT imaging was also performed in normal group.Biological distribution of^18F-Flurpiridaz was observed,and the ratio of radioactive uptake of^18F-Flurpiridaz between myocardium and adjacent tissues or organs was calculated.Image quality score and rest myocardial blood flow(rMBF)of 2 imaging tracers in normal group were measured and compared.MPI image quality score,cardiac function parameters such as summed rest score(SRS),myocardial infarction area percentage,total perfusion defect(TPD),and left ventricular ejection fraction(LVEF)of 2 imaging tracers were compared in myocardial infarction group.Data was analyzed by paired t test.Results In normal group,^18F-Flurpiridaz in the myocardium was clearly observed,with high radioactive uptake maintaining within 2 h postinjection.The radioactivity count ratios of left ventricular myocardium to cardiac pool,the lungs and liver were high(5.19-12.87,4.17-50.51,2.08-6.92).The quality of^18F-Flurpiridaz MPI images in both groups was excellent(10/10).The rMBF(ml·g-1·min-1)in different regions of left ventricle measured by^18F-Flurpiridaz and^13N-NH3·H2O imaging were not significantly different(left anterior descending:0.98±0.06 vs 0.92±0.^13;left circumflex:0.98±0.05 vs 0.88±0.12;right coronary artery:0.95±0.07 vs 0.88±0.15;left ventricle:0.96±0.07 vs 0.90±0.^13;t values:from-1.70 to-0.90,all P>0.05).There was no significant difference in SRS,myocardial infarction area percentage,TPD,rMBF or LVEF between^18F-Flurpiridaz and^13

关 键 词：心肌灌注显像 哒嗪类 氟放射性同位素 氨 正电子发射断层显像术 体层摄取术 X线计算机 猪 

分 类 号：R54[医药卫生—心血管疾病]