气相色谱法同时测定人参再造丸中龙脑、桂皮醛、丁香酚和麝香酮的含量  

Simultaneous Determination of Borneol,Cinnamaldehyde,Eugenol and Muscone in Renshenzaizao Pills by GC

作　　者：张博 智雪枝 田兰 包综方 陈睿 闫超[2] ZHANG Bo;ZHI Xue-zhi;TIAN Lan;BAO Zong-fang;CHEN Rui;YAN Chao(Shanghai Songjiang Institute for Food and Drug Control,Shanghai 201600,China;School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海市松江食品药品检验所,上海201600 [2]上海交通大学药学院,上海200240

出　　处：《现代中药研究与实践》2020年第1期64-67,共4页Research and Practice on Chinese Medicines

摘　　要：目的建立同时测定人参再造丸中龙脑、桂皮醛、丁香酚和麝香酮含量的气相色谱方法。方法采用HP-5毛细管柱(30 m×0.32 mm,0.25µm),程序升温;进样口温度250℃,氢火焰离子化检测器(FID),检测器温度280℃;载气为氮气,流速为1.5 ml/min,分流比10∶1,进样量为1µl。结果龙脑、桂皮醛、丁香酚和麝香酮分别在338.2~3382.4µg/ml(r=0.9999),162.4~1623.7µg/ml(r=0.9999),54.8~548.1µg/ml(r=0.9999),6.05~60.5µg/ml(r=0.9999)的范围内均呈良好的线性关系,平均回收率(n=6)分别为98.1%,97.4%,98.1%,98.9%。结论该方法完善了现行标准,可用于人参再造丸的质量控制。Objective To establish a GC method for the simultaneous determination of borneol,cinnamaldehyde,eugenol and muscone in Renshenzaizao pills.Methods A capillary column HP-5(30 m×0.32 mm,0.25μm)is used and the programmed temperature is set from 100℃ to 220℃.The detector is FID,inlet temperature is 250℃,the detector temperature is 280℃.The carrier gas is N2 and the flow rate of the carrier gas is 1.5 ml/min,the split ratio is 10∶1,the injection volume is 1μl.Results Borneol,cinnamaldehyde,eugenol and muscone are separated with a good linearity in the size ranges of 338.2~3382.4μg/ml for borneol,162.4~1623.7μg/ml for cinnamaldehyde,54.8~548.1μg/ml for eugenol,6.05~60.5μg/ml for muscone.The average recoveries(n=6)are 98.1%,97.4%,98.1%,98.9%,respectively.Conclusion The established method has improved the existing standards and it is suitable for the quality control of Renshenzaizao pills.

关 键 词：人参再造丸 龙脑 桂皮醛 丁香酚 麝香酮 含量测定 气相色谱法 

分 类 号：R284.1[医药卫生—中药学]