玉米瘤黑粉菌SG200效应蛋白PEP1表达及生物信息学分析  

Expression and bioinformatics analysis of effector protein PEP1 from Ustilago maydis SG200

作　　者：刘云云 李智敏[1] 程毅[1] 余永廷[1] 陈佳[1] 严准[1] LIU Yunyun;LI Zhimin;CHENG Yi;YU Yongting;CHEN Jia;YAN Zhun(Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha,Hunan 410205,China)

机构地区：[1]中国农业科学院麻类研究所

出　　处：《湖南农业大学学报（自然科学版）》2020年第1期28-32,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省重点研发计划项目(2016WK2030);中国农业科学院科技创新工程项目(CAAS–ASTIP–2015–IBFC09)

摘　　要：运用生物信息学手段分析pep1基因结构,并根据GenBank中玉米瘤黑粉菌pep1 DNA序列设计引物,从玉米瘤黑粉菌SG200的基因组中扩增得到了pep1基因全长,构建了pET-28a-pep1重组表达质粒,选用大肠埃希菌BL21(DE3)作为宿主菌,以1 mmol/L IPTG诱导表达。SDS-PAGE检测结果表明,诱导表达产物大小与理论值(20800)一致,说明pET-28a-pep1能够在大肠埃希菌BL21(DE3)中高效表达。PEP1 protein is one of the effector proteins of the Ustilago maydis.The study of PEP1 protein plays an important role for the new method development to combat the corn smut.In this study,the structure of pep1 gene was expressed,the resulting proteins were purified and analyzed by bioinformatics method.The recombinant expression plasmid pET-28 a-pep1 was constructed,and Escherichia coli BL21(DE3)was used as a host strain and induced with 1 mmol/L IPTG.The results of SDS-PAGE showed that the size of the induced expression product PEP1 was consistent with the theoretical value(20800),indicating that pET-28 a-pep1 is a capable strain to highly express the target protein in E.coli BL21(DE3).

关 键 词：玉米瘤黑粉菌 SG200菌株 效应蛋白PEP1 生物信息学分析 

分 类 号：Q786[生物学—分子生物学]