蓖麻RcGASA9基因克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of RcGASA9 Gene in Castor

作　　者：李孟建 李国瑞[1,2,3] 黄凤兰[1,2,3] 韩雯毓 风兰 陈永胜[1,2,3] Li Mengjian;Li Guorui;Huang Fenglan;Han Wenyu;Feng Lan;Chen Yongsheng(College of Life Sciences,Inner Mongolia University for Nationalities,Tongliao,028000;Inner Mongolia Industrial Engineering Research Center for Universities For Castor,Inner Mongolia University for Nationalities,Tongliao,028000;College of Agriculture,Inner Mongolia University for Nation alities,Tongliao,028000)

机构地区：[1]内蒙古民族大学生命科学学院,通辽028000 [2]内蒙古民族大学,内蒙古自治区高校蓖麻产业工程技术研究中心,通辽028000 [3]内蒙古民族大学农学院,通辽028000

出　　处：《分子植物育种》2019年第21期6981-6986,共6页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31460353);内蒙古自治区科技创新引导资金项目(KJCX15002);内蒙古民族大学科研项目(MDK2016008;MDK2017034;MDK2017035);草原英才创新团队项目;国家青年科学基金(31401418)共同资助

摘　　要：GASA蛋白是富含半胱氨酸的植物特有的一类小分子蛋白,GASA蛋白家族在赤霉素信号传导过程中具有重要的调控作用。本研究通过克隆RcGASA9基因以及对其进行相关的生物信息学分析;利用聚合酶链式反应(polymerase chain reaction, PCR)技术扩增该基因的完整阅读框,发现该基因片段长度为342 bp,编码113个氨基酸,分子量12 674.91 kD,等电点为9.45,不稳定指数为66.20。通过功能结构域预测发现RcGASA9蛋白属于GASA超大基因家族。同源性分析结果表明,与麻疯树亲缘关系最近,符合进化关系。这些分析结果为进一步研究RcGASA9蛋白功能提供理论依据。GASA protein is a plant-specific cysteine-rich protein family that plays an important role in GA hormone signal transduction. In this study, we cloned the gene encoding RcGASA9 protein, and bioinformatics analysis of RcGASA9 gene. The complete reading frame of this gene was cloned, the full length of this gene was342 bp, encoded 113 amino acids, molecular weight was 12 674.91 kD, isoelectric point was 9.45. The instability index was 66.20. It is predicted by functional domain that RcGASA9 protein belongs to the GASA superfamily family. Homology analysis showed high homology with Jatropha, and the result was consistent with species evolution. These results provide theoretical basis for further study of the functional role of RcGASA9 protein.

关 键 词：蓖麻(Ricinus communis) RcGASA9基因 基因克隆 生物信息学分析 

分 类 号：S565.6[农业科学—作物学] Q943.2[生物学—植物学]