利用RILs群体对早、晚季水稻穗部结构性状的QTL分析  

Dissection of QTL for Panicle Structure Traits by Using RILs Population under Early and Late Season in Rice

作　　者：刘进 姚晓云 王嘉宇[2] 李慧[1] 周慧颖 黎毛毛[1] Liu Jin;Yao Xiaoyun;Wang Jiayu;Li Hui;Zhou Huiying;Li Maomao(National Engineering Laboratory for Rice,Nanchang Sub-center,National Rice Improvement Center,Rice Research Institute,Jiangxi Academy of Agricultural Sciences,Nanchang,330200;Rice Research Institute,Shenyang Agricultural University,Shenyang,110866)

机构地区：[1]江西省农业科学院水稻研究所,国家水稻改良中心南昌分中心,水稻国家工程实验室,南昌330200 [2]沈阳农业大学水稻研究所,沈阳110866

出　　处：《分子植物育种》2019年第21期7094-7106,共13页Molecular Plant Breeding

基　　金：国家重点研发计划(2016YFD0100101-11);江西省青年科学基金(20171BAB214026);江西现代农业科研协同创新专项(JXXTCX201701-01);江西省农业科学院博士启动基金(20162CBS007)共同资助

摘　　要：为了发掘和利用新的高产相关的穗部结构性状的QTL,本研究以粳型超级稻‘龙稻5’和典型高产籼稻‘中优早8’杂交衍生的重组自交系群体为试验材料,在早、晚季生态环境下对水稻穗部结构性状进行QTL分析。早、晚季环境下穗部结构性状存在明显的变化,晚季环境下穗部产量性状优势明显,早、晚季环境下穗部结构性状间存在显著或极显著的相关性;共检测到61个穗部结构性状相关的QTL,仅13个QTL能在早、晚季环境下稳定表达,早、晚季环境下检测到QTL的特征值存在明显差异。水稻穗部结构性状QTL成簇分布在1号、3号、4号和10号染色体,其中QTL簇qGNC1和qGNC4b是两个重演性较好的多效性位点,多效性QTL簇qGNC4a和qGNC10是新发现的两个主效位点,此外,检测到23个加性效应QTL或非加性效应位点间的上位性互作位点。本研究结果丰富了穗部性状的遗传基础,并为两个新的主效QTL簇的深入研究提供了科学依据。To identify and use novel excellent higher yield gene of panicle structure traits, a set of RILs population derived from the cross between ’Longdao5’ and ’Zhongyouzao8’ was used to identify QTLs for panicle structure traits at early and late season environments. Under the ecological environment of early and late seasons,there were obvious changes of panicle structural traits, which the yeild and panicle traits of late season have a obvious dominant, and then there were significant correlations between early and late season for panicle structure traits. Sixty-one panicle structure traits related QTLs were detected, thirteen QTL were stably and reliably detected under two environments, therefore these QTL have significantly different between early and late seasons. These QTLs cluster were detected on the chromosome 1, 3, 4 and 10 with pleiotropic genetic overlap effect and stable expression, which these QTL cluster have significant effects on the panicle structure trait. Among these QTLs, two major QTL cluster qGNC1 and qGNC4 b were stable detected under multiple environments, two novel major QTL cluster q GNC4 a and q GNC10 were detected on the early and late season. In addition, epistatic interaction is one of the most important genetic component to regulate the panicle structure traits, and there have many epistatic QTL form between additive or non-additive effect locus. These results will provide information for genetic analysis of panicle structure traits, and also will provided a scientific basis for the further study of two novel major effect QTL clusters in rice.

关 键 词：水稻(Oryza sativa) 早季 晚季 穗部结构性状 QTL分析 

分 类 号：S511[农业科学—作物学]