基于功能微拓扑结构的细胞定位培养芯片  

Cell Positioning Culture Chip Based on Functional Micro Topological Substrate

作　　者：撒成花 薛艳 魏晨 梁浩彬 谢丽[6,7] 叶芳 SA Chenghua;XUE Yan;WEI Chen;LIANG Haobin;XIE Li;YE Fang(School of Mechanical Engineering, Northwestern Polytechnical University, Xi′an 710072, China;Key Laboratory of Micro/Nano Systems for Aerospace, Ministry of Education, Northwestern Polytechnical University, Xi′an 710072, China;China Hangfa Sichuan Gas Turbine Research Institute, Chengdu 610000, China;AVIC Shaanxi Aero Electric Co Ltd, Xi′an 713107, China;Shenzhen Mindray Bio-medical Electronics Co Ltd, Shenzhen 518057, China;School of Life Sciences, Northwestern Polytechnical University, Xi′an 710072, China;Key Laboratory of Space Bioscience and Biotechnology, Institute of Special Environmental Biophysics, Northwestern Polytechnical University, Xi′an 710072, China)

机构地区：[1]西北工业大学机电学院,陕西西安710072 [2]空天微纳系统教育部重点实验室,陕西西安710072 [3]中国航空航天四川燃气轮机研究院,四川成都610000 [4]中航陕西航空电气有限公司,陕西西安713107 [5]深圳市迈瑞生物医疗电子有限公司,广东深圳518057 [6]西北工业大学生命学院,陕西西安710072 [7]空间生物实验模拟技术国防重点学科实验室,陕西西安710072

出　　处：《西北工业大学学报》2020年第1期139-146,共8页Journal of Northwestern Polytechnical University

基　　金：陕西省自然科学基础研究计划(2018JM5146);111引智项目(B13044);陕西省科技统筹创新工程重点实验室项目资助

摘　　要：提出了一种功能微拓扑结构细胞培养芯片,芯片结构由具有细胞粘附特异性的微平台和微柱阵列交替排布而成,并采用具有良好生物相容性和化学稳定性的聚合物制备,因此该芯片具有对细胞规约能力强和定位稳定性好的优点。芯片性能验证结果表明,所设计的功能微拓扑结构芯片细胞定位率达到90%,芯片结构及尺寸稳定性好,为需要长期细胞培养的干细胞研究提供了重要的支撑工具。There are two main categories of cell positioning culture chips:chemical modification of surfaces and physical structure methods.The chemical methods have the advantage of providing a strong binding force,but the instability of chemical materials limits its application.The physical methods have the advantage of good stability for cell positioning,while the nonspecificity of physical structure weakens the ability to regulate cells.Here,we propose a functional micro topological chip.The chip is composed of micro-platform and micro-post array with different adhesion characteristics.At the same time,the chip is prepared by a polymer material with good biocompatibility and high chemical stability;therefore,it has the advantages of strong cell constraint ability and good localization stability.The result of chip performance verification shows that the cell localization rate of the functional micro topological chip is up to 90%,and the chip structure and dimension stability are good.The study provides an important supporting tool for the research of stem cells and their differentiation that need long-term cell culture.

关 键 词：细胞定位培养芯片 功能微拓扑结构 图形化 

分 类 号：Q658[生物学—生物物理学]