铜离子介导的石墨烯量子点荧光开-关高选择性检测谷胱甘肽  

Cu2+-mediated Fluorescence Switching of Graphene Quantum Dots for Highly Selective Detection of Glutathione

作　　者：李欣玉 张强 王妮 刘加军 王健[1] LI Xin-Yu;ZHANG Qiang;WANG Ni;LIU Jia-Jun;WANG Jian(Key Laboratory of Luminescent and Real-Time Analytical System(Southwest University),Chongqing Science and Technology Bureau,College of Pharmaceutical Sciences,Southwest University,Chongqing 400715,China;Demonstration Center for Experimental Pharmacy Education(Southwest University),College of Pharmaceutical Sciences,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学药学院发光与实时分析系统重庆市市级重点实验室,重庆400715 [2]西南大学药学院国家级药学实验教学示范中心,重庆400715

出　　处：《分析化学》2020年第3期339-346,共8页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21405123);重庆市自然科学基金项目(No.cstc2019jcyj-msxmX0279);中央高校基本科研业务费项目(No.XDJK2019AC002);西南大学药学院创新创业项目(No.YX2017-CXYB-10)资助~~

摘　　要：作为一种短肽,谷胱甘肽含有活性巯基,参与细胞内多种反应,因此,检测细胞中的谷胱甘肽具有重要意义。本研究合成了表面富含氨基的石墨烯量子点(GQDs),可与铜离子(Cu^2+)发生配位反应,聚集诱导荧光猝灭;而Cu^2+和谷胱甘肽具有更强的配位能力,促使Cu&2+从GQDs上解离下来,进而导致GQDs荧光的恢复。在pH 6.8的BR缓冲溶液中,谷胱甘肽可在20 min内将Cu^2+(250μmol/L)猝灭的GQDs(1μg/mL)的荧光恢复,且荧光信号的恢复程度与谷胱甘肽的浓度在20~500μmol/L范围内呈良好的线性关系,检出限为3.4μmol/L。本方法利用Cu^2+的开-关作用提高了选择性,可用于细胞裂解液中谷胱甘肽的检测。As a short peptide containing active thiol group,glutathione participates in many intracellular reactions,so it is of great significance to detect glutathione in cells.In this work,amino-rich graphene quantum dots were synthesized,which could coordinate with copper ions and yield aggregation-induced fluorescence quenching.However,copper ions and glutathione have stronger coordination ability,so that glutathione can promote the dissociation of copper ions from graphene quantum dots,and then lead to the fluorescence recovery of graphene quantum dots.In BR buffer media at pH 6.8,glutathione can gradually recover the fluorescence of graphene quantum dots(10g/mL)that is quenched by Cu^2+(2500mol/L),which can finish within 20 min.Additionally,recovery degree of fluorescence signal is linear to the concentration of glutathione in the range of 20-500μmol/L,and the detection limit is 3.4μmol/L.This method is applicable to the detection of glutathione in cell lysate by using the switch action of copper ion to improve the selectivity.

关 键 词：石墨烯量子点 谷胱甘肽 铜离子 荧光开-关 

分 类 号：O657.3[理学—分析化学] R318.08[理学—化学]