咖啡酸修饰单层石墨烯平面电极对烟酰胺腺嘌呤二核苷酸的选择性检测  

A Monolayer Graphene Platform Modified with Caffeic Acid for Selective Detection of Dihydronicotinamide Adenine Dinucleotide

作　　者：崔一鸣 何丹宇 李开 路丽琴[1] 罗红霞[1] CUI Yi-Ming;HE Dan-Yu;LI Kai;LU Li-Qin;LUO Hong-Xia(Department of Chemistry,Renmin University of China,Beijing 100872,China)

机构地区：[1]中国人民大学化学系

出　　处：《分析化学》2020年第3期378-388,共11页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21575160)资助~~

摘　　要：利用化学气相沉积法制得单层石墨烯,制备石墨烯平面电极(GPE),然后通过电化学沉积方法,将咖啡酸(CFA)固定在经过电化学活化的GPE上,用于烟酰胺腺嘌呤二核苷酸(NADH)的电化学检测。利用扫描电子显微镜、原子力显微镜、能谱、拉曼光谱、X射线光电子能谱以及红外光谱等方法对得到的CFA-GPE进行了表征。利用循环伏安法研究了NADH在CFA-GPE表面的电化学行为。在石墨烯和CFA的协同作用下,NADH氧化的过电位减小了0.43 V,峰电流明显增大,基于此构建了一种新型NADH传感器。此CFA-GPE传感器在NADH浓度为0.001~1200μmol/L范围内,响应电流与浓度呈良好的线性关系,检出限为0.52 nmol/L(S/N=3)。在同时含有NADH、谷胱甘肽和叶酸的混合溶液中,采用差分脉冲伏安法,3种物质的氧化峰可以很好地分开,从而实现了3种物质的选择性检测或同时检测。另外,本传感器具有很好的稳定性和抗干扰能力,并成功地应用于实际样本的检测。A monolayer graphene electrochemical platform based on chemical vapor deposition grown graphene was constructed for electrochemical detection of nicotinamide adenine dinucleotide(NADH).Caffeic acid(CFA)was immobilized on the surface of the activated graphene platform electrode(GPE)via electrodeposition.The CFA-GPE was characterized by scanning electron microscopy,atomic force microscopy,energy dispersive spectrometer,X-ray photoelectron spectroscopy,Raman spectra,and Fourier transform infrared spectra.The electrochemical behaviors of NADH were investigated by cyclic voltammetry.With the synergetic effect of graphene and CFA,the overpotential of NADH oxidation peak decreased by 0.43 V and its peak current increased significantly.Hence,a novel electrochemical sensor for selective detection of NADH was created.The broad linear response was presented by chronoamperometry,which was 0.001μmol/L to 1200μmol/L with a detection limit of 0.52 nmol/L(S/N=3).The simultaneous determination of NADH,glutathione and folic acid was realized by differential pulse voltammetry technique at CFA-GPE.In addition,the as-obtained sensor delivered good stability as well as high resistance to interference,and was successfully utilized in real sample detection.

关 键 词：化学气相沉积法单层石墨烯平面电极 咖啡酸 烟酰胺腺嘌呤二核苷酸 电化学传感 

分 类 号：O657.1[理学—分析化学]