Slug敲除促进γ-射线照射小鼠肠上皮细胞损伤的体内研究  

Slug knockout promotes γ-ray induced intestinal epithelial cell injury in mice in vivo

作　　者：李鹏[1] 朱庆丽[2] 宁亮[3] 焦学龙[3] 陈栋[3] 宫兴基[4] 贺东勇[4] LI Peng;ZHU Qingli;NING Liang;JIAO Xuelong;CHEN Dong;GONG Xingji;HE Dongyong(Department of Endocrinology,the Affiliated Hospital of Qingdao University,Shandong 266003;Department of thyroid surgery,the Affiliated Hospital of Qingdao University,Shandong 266003;Department of General Surgery,the Affiliated Hospital of Qingdao University,Shandong 266003;Department of Emergency Internal Medicine,the Affiliated Hospital of Qingdao University,Shandong 266003)

机构地区：[1]青岛大学附属医院内分泌科,山东266003 [2]青岛大学附属医院状腺外科,山东266003 [3]青岛大学附属医院普外科,山东266003 [4]青岛大学附属医院急诊内科,山东266003

出　　处：《交通医学》2020年第1期1-3,7,共4页Medical Journal of Communications

基　　金：山东省自然科学基金资助项目（2014ZRB01198）

摘　　要：目的:观察γ射线照射对Slug敲除C57BL/6小鼠肠粘膜上皮细胞损伤的影响及机制。方法:Slug敲除C57BL/6小鼠和非Slug敲除C57BL/6小鼠各12只,以8Gy 60Coγ射线全身一次性照射制作动物模型,分别在照射后第1、3、8天处死小鼠,取小肠组织4%多聚甲醛液固定作病理学检查;取非Slug基因敲除小鼠和Slug基因敲除小鼠各24只,予以8Gy 60Coγ射线全身一次性照射,分别在照射后第2、4和24 h处死,采用免疫组化法检测肠道细胞PUMA表达,TUNEL染色检测细胞凋亡。结果:8Gy 60Coγ射线照射后,Slug基因敲除小鼠死亡率91.67%,明显高于非基因敲除小鼠的25.00%,差异有统计学意义(P=0.004)。Slug敲除小鼠肠道细胞凋亡指数、PUMA阳性表达细胞数以及肠道损伤的严重程度均明显高于非敲除组。结论:Slug基因敲除加重γ射线引起的放射性肠道损伤。Objective:To observe the role and mechanisms of gamma ray(γ)irradiation on intestinal mucosa epithelial cells in C57BL/6 mice with Slug knockout.Methods:A total of 12 Slug knockout C57BL/6 mice and 12 non-Slug knockout C57BL/6 mice were selected.The mice were exposured to 8Gy 60Coγone-time to produce animal model,which was weighed and put to death at 1st day,3th day and 8th day,respectively.The jejunum and ileum was fixed in 4%paraformaldehyde fluid,paraffin embedding and sectioning.The histopathological changes were observed after HE staining.Expression of PUMA in intestinal mucosa epithelial cell was detected with immunohistochemical method.Cell apoptosis was detected by TUNEL staining.Results:8Gy 60Coγirradiation causes mice to lose spirit and reduces food intake.Two,four and five Slug knockout mice died at 1st day,3 th day and 8th day,respectively,and zero,one and two non-slug knockout C57BL/6 mice died at 1st day,3 th day and 8th day,respectively(P=0.004).The intestinal cell apoptosis index,PUMA expression and severity of intestinal injury in Slug knockout mice with 8Gy 60Coγwere significantly higher compared to non-Slug knockout mice.Conclusions:Slug knockout increases radioactive intestinal injury caused byγrays.

关 键 词：放射性肠道损伤 SLUG基因 PUMA蛋白 基因敲除 细胞凋亡 

分 类 号：R574.5[医药卫生—消化系统]