牛奶中氟喹诺酮类药物残留同步快速检测的酶联免疫吸附法研究  

Determination of Fluoroquinolones Residues in Milk byEnzyme-linked Immunosorbent Assay

作　　者：李燕君 谭梅[1] 岳秀英[2] 陆强[2] 吴晓岚[2] 吕晓华[1] LI Yan-jun;TAN Mei;YUE Xiu-ying;LU Qiang;WU Xiao-lan;LV Xiao-hua(West China School of Public Health and West China Fourth Hospital,Sichuan University,Chengdu 610041,China;Veterinary Drug Supervision of Sichuan Province,Chengdu 610041,China)

机构地区：[1]四川大学华西公共卫生学院/四川大学华西第四医院,四川成都610041 [2]四川省兽药监察所,四川成都610041

出　　处：《中国兽药杂志》2020年第1期46-52,共7页Chinese Journal of Veterinary Drug

基　　金：四川省科技计划(2019YFS00620);四川大学高校基本科研业务费研究专项项目(skqy201234)。

摘　　要：建立同时快速检测牛奶中环丙沙星、恩诺沙星、诺氟沙星、培氟沙星、氧氟沙星和洛美沙星六种氟喹诺酮类药物残留的酶联免疫吸附法。ELISA法的操作在20℃~25℃下进行,以50%抑制浓度、最低检测限反映方法的灵敏度,同时进行加标回收试验。结果表明,本文所建立的ELISA法的最低检测限为1.48μg/L,50%抑制浓度的范围在0.254~0.361μg/L;在环丙沙星、恩诺沙星、诺氟沙星、培氟沙星、氧氟沙星和洛美沙星药物添加浓度为5、25、50μg/L时的回收率为84.58%~115.92%,批内变异系数均小于15.0%,批间变异系数均小于16.0%。本方法简便快捷,灵敏度、准确度和精密度高,适用于牛奶中FQs残留的同时快速检测。An enzyme-linked immunosorbent assay was established for the simultaneous determination of six fluoroquinolone residues in milk,includes ciprofloxacin,enoxacin,norfloxacin,pefloxacin,ofloxacin and lomefloxacin.ELISA was performed at 20℃~25℃,the sensitivity of the method was reflected by 50%inhibition concentration and minimum detection limit,the recovery test was carried out at the same time.The results showed that the minimum detection limit of this method was 1.48μg/L,the range of 50% inhibitory concentration was 0.254~0.361μg/L;The recovery rate of ciprofloxacin,enoxacin,norfloxacin,pefloxacin,ofloxacin and lomefloxacin at 5,25 and 50μg/L was 84.58%~115.92%,the intra-batch variation coefficient was less than 15.0%,and the inter-batch variation coefficient was less than 16.0%.This method is simple and rapid,with high sensitivity,accuracy and precision.It is suitable for the rapid screening and detection of the residues of fluoroquinolones in milk.

关 键 词：酶联免疫吸附法 牛奶 氟喹诺酮类药物 残留 

分 类 号：S859.79[农业科学—临床兽医学]