SBBR反应器中耐冷微生物的驯化与识别  

Domestication and identification of cold-resistant bacteria in SBBR reactor

作　　者：吴涵 陈滢[1] 刘敏[1] 王淑莹[2] 张伟[3] WU Han;CHEN Ying;LIU Min;WANG Shuying;ZHANG Wei(College of Architecture and Environment,Sichuan University,Chengdu 610065,Sichuan,China;National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology,Beijing University of Technology,Beijing 100124,China;State Key Laboratory of Polymer Materials Engineering,Polymer Research Institute at Sichuan University,Chengdu 610065,Sichuan,China)

机构地区：[1]四川大学建筑与环境学院,四川成都610065 [2]北京工业大学城镇污水深度处理与资源化利用技术国家工程实验室,北京100124 [3]四川大学高分子研究所高分子材料工程国家重点实验室,四川成都610065

出　　处：《化工学报》2020年第2期766-776,共11页CIESC Journal

基　　金：四川大学大学生创新训练计划(C2019104263);城镇污水深度处理与资源化利用技术国家工程实验室开放基金;2018年度中央高校基本科研业务费专项资金

摘　　要：以模拟生活污水为处理对象,三种不同填料为生物膜载体,运行A、B、C三个SBBR,通过逐步降温(25、20、15、10、6和5℃)培养驯化处理低温污水的生物膜。运行251周期,三个反应器COD出水浓度均低于50 mg/L;B反应器对氨氮去除效果优于A和C。特别在低温5℃时,A、B和C三个反应器氨氮平均出水浓度分别为14.1、3.79和14.1 mg/L。高通量测序结果显示,5℃时,三个反应器中均以降解有机物的微生物为主;只有B反应器中驯化富集出了嗜冷硝化菌(Candidatus Nitrotoga);而其他有硝化作用的菌属在B反应器中的丰度也高于A和C反应器;不利于脱氮的类固氮菌(Elstera)在A和C反应器中丰度较高。此结果可从微生物角度解释三个反应器处理效果的差异性,表明B反应器处理低温污水有显著优势,其所用填料可结合低温菌进一步开发利用。Taking simulated domestic sewage as the treatment object,three different fillers are biofilm carriers,and three SBBRs of A,B and C are operated,and the biofilm of low temperature sewage is domesticated and treated by gradual cooling(25,20,15,10,6 and 5℃).During the 251 operating cycles,the COD effluent concentration of the three reactors was less than 50 mg/L.The ammonium removal efficiency of the B reactor was better than A and C.At 5℃,the ammonium concentration of effluent of A,B and C were 14.1,3.79 and 14.1 mg/L,respectively.The results of high-throughput sequencing showed that the dominant microbial species in SBBR were mainly those can degrade organic matter at 5℃.Psychrophilic nitrifying bacteria(Candidatus Nitrotoga)were found only in B reactor.The abundance of other common nitrifying bacteria in the B reactor is higher than which in the A and C reactors.The nitrogen-fixing bacteria(Elstera),which adverse to remove ammonium,has the minimal distribution in the B reactor.These results can explain the difference of the pollutant removal of the three reactors from point of view of microorganism,which shows that B reactor has significant advantages in treating low temperature wastewater.

关 键 词：废水 生物膜 低温 高通量测序 微生物群落结构 

分 类 号：X703[环境科学与工程—环境工程]