利用直扩PCR试剂盒检测小麦多效抗病基因  

Detection of Pleiotropic Disease Resistant Genes in Wheat by Direct PCR Kit

作　　者：李玮[1] 宋国琦[1] 李玉莲[1] 张淑娟[1] 张荣志[1] 高洁[1] 李吉虎 李根英[1] Li Wei;Song Guoqi;Li Yulian;Zhang Shujuan;Zhang Rongzhi;Gao Jie;Li Jihu;Li Genying(Crop Research Institute,Shandong Academy of Agricultural Sciences/Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow&Huai River Valley,Ministry of Agriculture/National Engineering Laboratory for Wheat&Maize,Jinan 250100,China)

机构地区：[1]山东省农业科学院作物研究所/农业部黄淮北部小麦生物学与遗传育种重点实验室/小麦玉米国家工程实验室

出　　处：《山东农业科学》2020年第2期15-18,共4页Shandong Agricultural Sciences

基　　金：山东省重点研发计划项目(2018GNC110033);山东省农业良种工程项目(2019LZGC001-1-4);国家自然科学基金项目(31701428);山东省农业科学院农业科技创新工程项目(CXGC2019G02)

摘　　要：为了明确直扩PCR在小麦多效抗病基因检测中的效果,促进廉价、快捷的直扩PCR技术在小麦分子育种中的应用,本研究通过对聚合三个多效抗病基因Sr2、Lr34、Lr67的BC2单株进行直扩PCR检测,筛选到了三个基因聚合的杂合单株,证明了直扩PCR的有效性,并讨论了直扩PCR的优缺点和影响因素,为直扩PCR技术在作物分子育种中的推广应用提供参考。To clarify the effect of direct PCR in detecting pleiotropic disease resistant genes in wheat,and promote the utilization of inexpensive and rapid direct PCR technology in wheat molecular breeding,the BC2 progeny were detected by direct PCR. The results showed that the heterogeneous individuals with three pleiotropic disease resistant genes Sr2,Lr34 and Lr67 were selected by direct PCR which indicated the effectiveness of direct PCR. The advantages,disadvantages and impact factors of direct PCR were also discussed. This study would provide references for popularization and application of direct PCR in crop molecular breeding.

关 键 词：小麦 直扩PCR试剂盒 多效抗病基因 分子标记 

分 类 号：S512.1[农业科学—作物学] Q781[生物学—分子生物学]