肺结核患者外周血单个核细胞NFκB1与LncRNA-PACER的表达关系研究  

Study on the relationship between the expression of NFκB1 and LncRNA-PACER in peripheral blood mononuclear cells of patients with pulmonary tuberculosis

作　　者：黄东轩 何朝文 廖毅力 彭剑锋 杨帆 曹亚辉 黄冬生 HUANG Dong-Xuan;HE Zhao-Wen;LIAO Yi-Li;PENG Jian-Feng;YANG Fan;CAO Ya-Hui;HUANG Dong-Sheng(Department of respiratory and critical care medicine,Shenzhen longhua district central hospital.ShenZhen Longhua 518110;Department of obstetrics,Shenzhen Maternity&Child Healthcare Hospital.ShenZhen Futian 518028)

机构地区：[1]深圳市龙华区中心医院呼吸与危重症医学科,深圳龙华518110 [2]深圳市妇幼保健院产科,深圳福田518028

出　　处：《海南医学院学报》2020年第4期285-289,共5页Journal of Hainan Medical University

基　　金：深圳市科技计划(JCYJ20180306172419505)~~

摘　　要：目的:探讨肺结核患者外周血单个核细胞(PBMCs)中核转录因子kappa B1(NFκB1)与长链非编码RNA PACER(LncRNA-PACER)的表达关系。方法:收集本院确诊的肺结核患者40例(肺结核组)及同期健康体检者40例(对照组),采用酶联免疫吸附(ELISA)法检测血清中肿瘤坏死因子-α(TNF-α)、白介素(IL)-6和IL-8水平;实时荧光定量PCR法检测PBMCs中LncRNA-PACER、NFκB1 mRNA表达;Western blot检测PBMCs中NFκB1、COX2蛋白表达;Pearson法分析肺结核患者PBMCs中LncRNA-PACER、NFκB1表达的相关性;分析肺结核患者PBMCs中LncRNA-PACER、NFκB1表达与临床病理特征的关系。结果:与对照组相比,肺结核组血清中TNF-α、IL-6和IL-8表达均显著升高(P<0.05),PBMCs中LncRNA-PACER、NFκB1 mRNA及蛋白、COX2蛋白表达均显著升高(P<0.05)。PBMCs中LncRNA-PACER、NFκB1蛋白表达水平与患者肺部病灶数量、肺部空洞有关(P<0.05),肺结核患者PBMCs中LncRNA-PACER表达与NFκB1 mRNA表达呈正相关(r=0.873,P<0.05)。结论:肺结核患者PBMCs中NFκB1、LncRNA-PACER表达显著升高,二者呈正相关,均与肺结核发生发展密切相关。Objective: To investigate the expression relationship between nuclear transcription factor kappa B 1(NFκB1) and long non-coding RNA PACER(LncRNA-PACER) in peripheral blood mononuclear cells(PBMCs) of patients with pulmonary tuberculosis. Methods: From February 2018 to March 2019, 40 patients with pulmonary tuberculosis(tuberculosis group) and 40 healthy persons(control group) were collected, the levels of TNF-α, IL-6 and IL-8 in serum were detected by enzyme-linked immunosorbent assay(ELISA);the expressions of LncRNA-PACER and NFκB1 mRNAs in PBMCs were detected by real-time fluorescence quantitative PCR;Western blot was used to detect the expressions of NFκB1 and COX 2 in PBMCs;Pearson method was used to analyze the expressions of LncRNA-PACER and NFκB1 in PBMCs of patients with pulmonary tuberculosis;and the expressions of LncRNA-PACER and NFκB1 in PBMCs of patients with pulmonary tuberculosis were analyzed. Results: Compared with the control group, the expressions of TNF-α, IL-6 and IL-8 in the serum of patients with pulmonary tuberculosis was significantly increased(P<0.05), and the expressions of LncRNA-PACER, NFκB1 mRNAs, proteins and COX-2 protein in PBMCs were significantly increased(P<0.05). The expressions of LncRNA-PACER and NFκB1 proteins in PBMCs were related to the number of pulmonary lesions and pulmonary cavity(P<0.05), and there was a positive correlation between the expression of LncRNA-PACER and the expression of NFκB1 mRNA in PBMCs of patients with pulmonary tuberculosis(r=0.873, P<0.05). Conclusions: The expressions of NFκB1 and LncRNA-PACER in PBMCs of patients with pulmonary tuberculosis are significantly increased, they are positively correlated and both of them are related to the occurrence and development of pulmonary tuberculosis.

关 键 词：肺结核 外周血单个核细胞 核转录因子kappa B 1 长链非编码RNA PACER 

分 类 号：R521[医药卫生—内科学]