基于网络药理学探讨益智仁-乌药治疗糖尿病肾病的有效成分及作用机制  

Exploration on the Effective Components and Mechanism of Alpinia Oxyphylla-Radix Linderae in the Treatment of Diabetic Nephropathy Based on Network Pharmacology

作　　者：姚宇剑 倪雅丽 武素 肖曼 袁勇[4] 罗丽玲 谢毅强[4] 李丽 YAO Yujian;NI Yali;WU Su;XIAO Man;YUAN Yong;LUO Liling;XIE Yiqiang;LI Li(Grade 2017 Graduate,Guizhou University of Traditional Chinese Medicine,Guizhou Province,Guiyang 550025,China;Pharmacy Department,the Second People’s Hospital of Hainan Province,Hainan Province,Wuzhishan 572200,China;Endocrinology Department,Hainan Provincial Hospital of Traditional Chinese Medicine,Hainan Province,Haikou 570203,China;Department of Traditional Chinese Medicine,Hainan Medical University,Hainan Province,Haikou 571199,China)

机构地区：[1]贵州中医药大学中医学硕士研究生2017级,贵州贵阳550025 [2]海南省第二人民医院药剂科,海南五指山572200 [3]海南省中医院内分泌科,海南海口570203 [4]海南医学院中医学院,海南海口571199

出　　处：《中国中医药现代远程教育》2020年第3期59-63,共5页Chinese Medicine Modern Distance Education of China

基　　金：海南省自然科学基金【No.2019CXTD407】;海南省大学生创新创业项目【No.201911810020】

摘　　要：目的基于网络药理学探讨益智仁-乌药治疗糖尿病肾病的有效成分及作用机制。方法运用多个在线数据库查找并筛选益智仁和乌药的活性成分、益智仁和乌药活性成分作用的相关靶点、糖尿病肾病相关的基因;通过UniProt数据库查询靶点对应的基因名;运用Cytoscape 3.2.1构建益智仁-乌药药对化合物对应靶基因网络;运用韦恩图筛选活性成分对应靶点与疾病相关基因的交集基因,并将交集的基因构建蛋白互作关系(PPI)网络;最后用DAVID的数据库对交集的基因做GO功能富集分析和KEGG信号通路富集分析,并将GO与KEGG的结果进行可视化处理。结果共筛选出10个潜在活性成分和相应的108个靶蛋白;益智-乌药药对潜在活性成分靶点与DN的相关基因共有36个交集基因;交集蛋白之间相互作用关联度排名前十的包括:AR与NCOA2、NCOA2与PPARG及NR3C1与NCOA2等;分子功能(MF)的条目有15条、细胞成分(CC)的条目有12条、生物过程(BP)的条目有48条及信号通路有19条(P<0.05)。结论益智仁-乌药通过多成分、多靶点及多环节的协同作用治疗DN。Objective To explore the effective components and mechanism of Alpinia Oxyphylla-Radix Linderae in the treatment of diabetic nephropathy based on network pharmacology.Methods Using multiple online databases to search and screen the active components of Alpinia Oxyphylla and Radix Linderae,the related targets of the active ingredients of Alpinia oxyphylla and Lindera aggregata,and the genes related to diabetic nephropathy,Query the gene name corresponding to the target through the UniProt database,Cytoscape 3.2.1 was used to construct the target gene network of Alpinia Oxyphylla and Radix Linderae for the compound.Using Venn diagram to screen for the intersection of the active ingredient target and the disease-related gene,and the intersection of the gene-construction protein interaction relationship(PPI)network,finally,the DAVID database was used to perform GO function enrichment analysis and KEGG signal pathway enrichment analysis on the intersection genes,and the results of GO and KEGG were visualized.Results A total of 10 potential active components and 108 corresponding target proteins were screened.There are 36 intersecting genes between target of potential active ingredient of Alpinia Oxyphylla and Radix Linderae and DN.The top ten interactions between intersection proteins include:AR and NCOA2,NCOA2 and PPARG,and NR3C1 and NCOA2,etc.There are 15 entries for molecular function(MF),12 entries for cellular components(CC),48 entries for biological processes(BP),and 19 entries for signaling pathways(P<0.05).Conclusion Alpinia oxyphylla-Radix Linderae can treat DN by synergistic action of multiple components,multiple targets and multiple links.

关 键 词：益智仁 乌药 糖尿病肾病 网络药理学 消渴 水肿 

分 类 号：R285.5[医药卫生—中药学]