白细胞介素-6抑制剂对银屑病模型大鼠背部皮肤组织的影响及作用机制  

Effect of IL-6 inhibitor on the skin tissue of psoriasis model rats and its mechanism

作　　者：魏荣 吴斌[1] 吕静[1] WEI Rong;WU Bin;Lv Jing(Department of Dermatology,Renhe Hospital Affiliated to Three Gorges University,Yichang 443001,Hubei,China)

机构地区：[1]三峡大学附属仁和医院皮肤科

出　　处：《中国老年学杂志》2020年第4期856-860,共5页Chinese Journal of Gerontology

基　　金：三峡大学青年科学基金(KJ2012A016)

摘　　要：目的研究白细胞介素(IL)-6抑制剂对银屑病模型大鼠背部皮肤组织的影响及作用机制。方法选取30只SD健康大鼠,随机分为空白对照组、银屑病组和IL-6抑制剂组各10只,建立银屑病模型,建模成功后,IL-6抑制剂组大鼠腹腔注射5 mg/kg IL-6抑制剂,空白对照组、银屑病组腹腔注射等剂量的生理盐水。在对大鼠最后一次给药后2 d观察大鼠变化,采集大鼠背部皮肤组织行苏木素-伊红(HE)染色、背部皮损表皮厚度检测、Baker病理评分、炎性细胞计数及检测背部皮肤组织中IL-17、IL-22、IL-23表达水平、IL-6受体/Janus激酶/信号转导和转录激活因子(IL-6R/JAK/STAT3)通路因子mRNA表达量。结果银屑病组、IL-6抑制剂组大鼠Baker评分、炎症细胞浸润数、表皮厚度、背部皮肤组织中IL-17、IL-22、IL-23表达水平、IL-6R、JAK1、STAT3、CD80、CCL5mRNA表达量均显著高于空白对照组(P<0.05);IL-6抑制剂组大鼠Baker评分、炎症细胞浸润数、表皮厚度、背部皮肤组织中IL-17、IL-22、IL-23表达水平、IL-6R、JAK1、STAT3、CD80、CCL5mRNA表达量均显著低于银屑病组(P<0.05)。结论IL-6抑制剂可有效抑制银屑病大鼠背部皮肤组织角质增殖、炎症反应,其作用机制可能与调控IL-6R/JAK/STAT3通路转导因子有关。Objective To study the effect and mechanism of IL-6 inhibitor on the back skin tissue of psoriasis model rats.Methods Thirty SD healthy rats were randomly divided into blank control,psoriasis and IL-6 inhibitor groups,10 rats in each group.Psoriasis model was established.After successful modeling,rats in IL-6 inhibitor group were injected with 5 mg/kg IL-6 inhibitor intraperitoneally,rats in blank control and psoriasis groups were injected with normal saline.The changes of rats were observed in 2 days after the last administration.HE staining,epidermal thickness measurement,Baker pathological score,inflammatory cell count and the expression of IL-17,IL-22,IL-23 in the back skin of rats,IL-6 receptor/Janus kinase/signal transduction and activator of transcription(IL-6R/JAK/STAT3)pathway factor mRNA expression were detected.Results Baker score,infiltration number of inflammatory cells,epidermal thickness,expressions of IL-17,IL-22,IL-23,IL-6R mRNA,JAK1 mRNA,STAT3 mRNA,CD80 mRNA and CCL5 mRNA in psoriasis group and IL-6 inhibitor group were higher than those in blank control group(P<0.05).Baker score,infiltration number of inflammatory cells,epidermal thickness,expression levels of IL-17,IL-22,IL-23,IL-6R mRNA,JAK1 mRNA,STAT3 mRNA,CD80 mRNA and CCL5 mRNA in back tissue of IL-6 inhibitor group were lower than those of psoriasis group(P<0.05).Conclusions IL-6 inhibitors could effectively inhibit keratinocyte proliferation and inflammation in psoriatic rat dorsal skin.Its mechanism might be related to the regulation of IL-6R/JAK/STAT3 pathway transduction factors.

关 键 词：白细胞介素-6抑制剂 银屑病 背部皮肤组织 白细胞介素-6受体/Janus激酶/信号转导和转录激活因子通路 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]