RP-HPLC法测定头孢羟氨苄干混悬剂中头孢羟氨苄的含量及有关物质  

RP-HPLC Determination of Cefadroxil Dry Suspension and its Related Substances

作　　者：张锋 ZHANG Feng(College of Life Sciences,ShanXi Agricultural University,Taigu 030801,Shanxi,China)

机构地区：[1]山西农业大学生命科学学院

出　　处：《海峡药学》2019年第12期67-71,共5页Strait Pharmaceutical Journal

摘　　要：目的建立测定头孢羟氨苄干混悬剂中头孢羟氨苄的含量及有关物质的方法。方法采用Waters C18色谱柱(3.9mm×150mm,5μm);以0.05mol·L-1磷酸钾缓冲溶液(用10mol·L-1氢氧化钠溶液调节pH至5.5)-乙腈(98∶2)为流动相;流速:1.0mL·min-1;检测波长:230nm;柱温:40℃;进样量:10μL。结果反相高效液相色谱法测定头孢羟氨苄干混悬剂中头孢羟氨苄的线形范围为1.4~4.2μg;日内RSD为0.1%(n=6)。结论本方法可用于头孢羟氨苄干混悬剂含量及其有关物质的测定,方法准确、快速、灵敏、简便。OBJECTIVE A method was established for the determination of Cefadroxil dry suspension and its related substances.METHODS The separation was performed on a Waters C18 column(3.9 mm×150 mm,5μm).The mobile phase consisted of 0.05 mol·L-1 kalium phosphate buffer(adjusted pH to 5.5 with 10 mol·L-1 NaOH solution)-acetonitrile(98∶2).The flow rate was 1.0 mL·min-1.The determination wavelength was at 230 nm.The chromatography was conducted at 40℃.The injection was 10μL.RESULTS The calibration curves were linear between 1.4-4.2μg(Y=635629 X-112131,r=1.0000);Intra-day RSD was 0.1%(n=6).CONCLUSION The method is accurate,rapid,sensitive,simple and is suitable for the determination of Cefadroxil dry suspension and its related substances.

关 键 词：RP-HPLC 头孢羟氨苄 混悬剂 含量 

分 类 号：R927[医药卫生—药学]