角额壁蜂共生放线菌分离、鉴定及其发酵产物抗菌活性研究  

Isolation and identification of symbiotic actinomycetes associated with meson bee Osmia cornifrons and the antibacterial and antifungal activity of their fermentation broth

作　　者：张修梅 王哲[2] 谢卫东[2] 刘旭[2] 任玉红 初晓艺 ZHANG Xiu-Mei;WANG Zhe;XIE Wei-Dong;LIU Xu;REN Yu-Hong;CHU Xiao-Yi(Shandong Drug and Food Vocational College,Weihai 264210,Shandong Province,China;Shandong University,Weihai 264209,Shandong Province,China)

机构地区：[1]山东药品食品职业学院,山东威海264210 [2]山东大学,山东威海264209

出　　处：《环境昆虫学报》2020年第1期157-169,共13页Journal of Environmental Entomology

基　　金：山东省自然科学基金(2014ZRE27019)

摘　　要：近年来,昆虫与共生菌的关系受到越来越多的关注,研究昆虫共生菌的种类及生物学功能具有重要意义。对从烟台、威海采集的角额壁蜂及其巢室进行放线菌的分离、纯化和16S rRNA序列测序并构建系统发育树,分别采用菌块对峙法和纸片扩散法测定菌株及其发酵液抗真菌和抗细菌活性。共计得到37株放线菌,鉴定结果表明共生菌菌株分别归属于7个属:链霉菌属26株,小单孢菌属3株,拟诺卡氏菌属2株,珊瑚状放线菌属1株,马杜拉放线菌属3株、假诺卡氏菌属1株和疣孢菌属1株。Streptomyces sp.OC1611-8A和Streptomyces sp.R1706-8菌体抗真菌作用较强,对杨生盾壳霉和云南刺盘孢抑菌圈直径更是达到了25~32 mm;Micromonospora sp.OC1403-4发酵液对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌均有较好的抗菌活性,抑菌圈直径达到了16~19 mm。本研究为进一步分离角额壁蜂共生菌的发酵产物,鉴定具有新型结构的抗菌化合物提供了参考。In recent years,more and more attention has been paid to the relationship between insects and symbiotic bacteria,and it is of significance to identify the symbiont species and biological functions.Symbiotic actinomycetes were screened from Osmia cornifrons and their nests collected in Yantai and Weihai,16S rRNA gene sequences of the isolates were analyzed and phylogenetic relationships were evaluated by constructing phylogenetic tree.Inhibitory activity of the strains and their fermentation broth were investigated and measured by the mash screen method and disk diffusion method,respectively.A total of 37 strains of actinobacteria were isolated and assigned to 7 different genera.Among them,26 strains belonged to Streptomyces,and the other 11 strains to Micromonospora(3),Nocardiopsis(2),Actinocorallia(1),Actinomadura(3),Pseudonocardia(1)and Verrucosispora(1).Streptomyces sp.OC1611-8A and R1706-8 had good antifungal effects on the tested fungi,especially Coniothyrium celtidicola and Colletotrichum yunnanse with inhibitory diameters of 25~32 mm;the fermentation liquid of Micromonospora sp.OC1403-4 had strong bioactivity against Escherichia coli,Staphylococcus aureus and Bacillus subtilis with inhibition zones 16~19 mm.This study could provide a reference for the discovery of bioactive compounds with new structures based on the further separation and purification of their fermentation products.

关 键 词：角额壁蜂 共生放线菌 发酵产物 分离鉴定 活性 

分 类 号：Q968.1[生物学—昆虫学] S476[农业科学—农业昆虫与害虫防治]