基于L-肉碱通路探讨灵归方对弱精子症大鼠生殖系统保护作用的实验研究  

Linggui Fang protects the reproductive system of asthenospermia rats: An experimental study based on the L-carnitine pathway

作　　者：晏斌 王福[1] 高庆和[1] 张继伟[1,2] 张修举 余国今[1] 邱俊峰[1] 刘煜[1] 刘胜京 郭军 YAN Bin1,2;WANG Fu;GAO Qing-he;ZHANG Ji-wei;ZHANG Xiu-ju;YU Guo-jin;QIU Jun-feng;LIU Yu;LIU Sheng-jing;GUO Jun(Department of Andrology,Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China;Graduate School,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院西苑医院男科,北京100091 [2]中国中医科学院研究生院,北京100700

出　　处：《中华男科学杂志》2019年第12期1113-1117,共5页National Journal of Andrology

基　　金：国家自然科学基金面上项目(81473527);国家自然科学基金青年项目(81703929)~~

摘　　要：目的:探讨灵归方对奥硝唑(ORN)所致弱精子症大鼠生殖系统保护作用及其可能机制。方法:将40只体重200~230 g的雄性SD大鼠随机将其分为空白组、模型组、灵归方组、左卡尼汀组各10只。模型组:予400 mg/kg ORN灌胃;灵归方组:予灵归方浓缩液,按17.5 g生药/kg体重+400 mg/kg ORN灌胃;左卡尼汀组:予左卡尼汀100 mg/kg+400 mg/kg ORN灌胃;空白组:予等量0.5%羧甲基纤维素钠(CMC2Na);均1次/d,连续灌胃4周后,检测各组大鼠精液质量、附睾中L-肉碱的含量,大鼠附睾OCTN2 mRNA的表达并观察睾丸组织病理。结果:与模型组相比,各组精子浓度无统计学差异(P>0.05),前向运动精子(PR)和前向+非前向运动(NP)精子百分率均高于模型组,差异有统计学意义(P<0.01)。与模型组相比,各组附睾的肉碱含量均提高,有统计学差异(P<0.01)。灵归方组、左卡尼汀组OCTN2 mRNA表达量明显高于模型组,有统计学差异(P<0.05)。睾丸病理观察结果发现,与空白组比较,模型组异常结构生精小管明显增多,排列不整齐,生精小管管腔缩小加重,管腔内精子及精子细胞数量减少,空腔型生精小管明显增多,生精小管内部各级生精细胞缺失,排列层次紊乱,上皮部分变性并出现空泡征;左卡尼汀组及灵归方组大鼠睾丸组织结构趋于正常,生精小管中各级生精细胞排列基本规整,生精小管结构基本正常。结论:①ORN可诱导大鼠产生弱精子症,且与降低附睾L-肉碱含量可能有关;②灵归方可以改善奥硝唑诱导的弱精子症大鼠精液活力;③灵归方可以改善ORN诱导睾丸的病理组织结构;④灵归方可以提高附睾中OCTN2 mRNA在附睾中的表达,其可能与提高附睾肉碱浓度有关。Objective: To explore the protective effect of the Chinese medicinal prescription Linggui Fang(LGF) on the reproductive system of the ornidazole-induced asthenospermia(AS) rat and its possible action mechanisms. Methods: Forty male SD rats weighing 200-230 g were equally randomized into four groups, blank control, AS model control, LGF treatment and L-carnitine(LC) intervention. The AS models were made in the latter three groups by intragastrical administration of ornidazole at 400 mg/kg. Meanwhile, the rats in the LGF group were treated intragastrically with LGF at 17.5 g/kg, those in the LC group with LC at 100 mg/kg, and the control animals with 0.5% sodium carboxymethylcellulose(CMC-Na), all once a day for 4 successive weeks. Then, all the rats were sacrificed for examination of the semen parameters, determination of the LC content and OCTN2 mRNA expression in the epididymis and observation of the histopathological changes in the testis. Results: Compared with the AS model controls, the rats in the other groups showed significantly higher percentages of progressively motile sperm and total motile sperm(P < 0.01) as well as a higher LC content in the epididymis(P < 0.01), but no statistically significant difference in sperm concentration(P > 0.05). The expression of OCTN2 mRNA was remarkably upregulated in the LGF and LC groups in comparison with that in the AS model control(P < 0.05). Compared with the rats in the blank control group, the AS model controls exhibited markedly increased morphologically abnormal seminiferous tubules, irregularly arranged, with narrowed lumens and reduced numbers of sperm and sperm cells, as well as significantly increased hollow seminiferous tubules with deficient and disorderly arranged spermatogenic cells and partial epithelial degeneration and vacuolization. Those in the LGF and LC groups, however, manifested almost normal testicular histomorphology, with basically regular arrangement of different layers of seminiferous tubules. Conclusion: ①Ornidazole induces AS in rats by

关 键 词：L-肉碱 弱精子症 灵归方 奥硝唑 

分 类 号：R698.2[医药卫生—泌尿科学]