简便烘干在水稻叶片DNA大量提取中的应用  

Application of Simple Drying in Extraction of Large Amount of DNA from Rice Leaves

作　　者：田孟祥 宫彦龙 张时龙 雷月 何友勋 李佳丽 余本勋 张大双 叶永印 闫志强 TIAN Mengxiang;GONG Yanlong;ZHANG Shilong;LEI Yue;HE Youxun;LI Jiali;YU Benxun;ZHANG Dashuang;YE Yongyin;YAN Zhiqiang(Bijie Institute of Agricultural Science,Bijie 551714,China;Guizhou Rice Institute,Guiyang 550006,China)

机构地区：[1]毕节市农业科学研究所,贵州毕节551714 [2]贵州省水稻研究所,贵州贵阳550006

出　　处：《河南农业科学》2020年第2期52-57,共6页Journal of Henan Agricultural Sciences

基　　金：国家重点研发计划项目(2017YFD0100402);贵州省科技成果应用及产业化计划项目(黔科合成果[2018]4306);毕节市科技支撑计划项目(毕科合字[2016]20号)

摘　　要：为探索烘干法替代液氮冷冻法研磨水稻叶片提取DNA的可行性,首先比较50℃低温烘干法与液氮冷冻法研磨水稻叶片提取DNA的扩增效果,然后进一步分析不同温度(50、60、70、80、90℃)烘干水稻叶片研磨提取DNA的扩增效果,筛选最佳的烘干温度。结果表明,水稻叶片在50~70℃下烘干磨碎所提取的DNA均能进行有效扩增,以50℃效果最佳,其与液氮冷冻法研磨叶片提取DNA的扩增效果无明显差异。水稻叶片烘干法具有操作简单、费用低廉及实用性强等诸多优点,在DNA大量提取中可较好地替代液氮冷冻法对叶片进行制备,具有一定的推广应用价值。In order to explore the feasibility of drying method instead of liquid nitrogen freezing method to grind rice leaves to extract DNA,firstly the amplification effects of DNA extracted from grinded rice leaves by low temperature drying under 50℃and liquid nitrogen freezing were compared,and then the amplification effects of DNA extracted from leaf grinded by drying method under different temperature(50,60,70,80,90℃)were further analyzed to screen the best drying temperature.The results showed that the DNA extracted from leaf grinded by drying method under 50—70℃could be effectively amplified,the effect was the best under 50℃,and there was no significant difference between the amplification effects of the DNA extracted from leaf grinded by drying method and liquid nitrogen freezing method.The drying method of rice leaves had many advantages,such as simple operation,low cost and strong practicability,which could replace the liquid nitrogen freezing method to prepare the leaves in the large-scale extraction of DNA,and had good popularization and application value.

关 键 词：水稻 叶片 简便烘干法 DNA大量提取 液氮冷冻法 

分 类 号：S511[农业科学—作物学]