过山蕨水提物的化学成分分离鉴定  

Chemical Constituents of Water Extract from Camptosorus sibiricus

作　　者：纪梦颖 陶移文[1] 乔晓旭 高慧[2] 杜焕嫦 吴鹏[2] 王玉青 JI Meng-ying;TAO Yi-wen;QIAO Xiao-xu;GAO Hui;DU Huan-chang;WU Peng;WANG Yu-qing(Guangzhou Medical University,Guangzhou 511436,China;Guangzhou University of Chinese Medicine,Guangzhou 510006,China)

机构地区：[1]广州医科大学,广州511436 [2]广州中医药大学,广州510006

出　　处：《中国实验方剂学杂志》2020年第2期124-128,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省自然科学基金项目(2016A030313588);2017年海洋和渔业发展专项(科技攻关与研发)(A201701C07);国家自然科学基金青年科学基金项目(81503224)

摘　　要：目的:过山蕨为铁角蕨科过山蕨属植物过山蕨(Camptosorus sibiricus)的地上全草部位,是一种民间常用药,目前关于过山蕨的生物活性研究主要集中在过山蕨总黄酮上,对其化学成分的研究尚有不足,药效成分也不能完全确认。该实验对过山蕨的化学成分进行系统分离,从药效物质基础的角度探寻其作为过山蕨补充药物来源的可能性,为进一步的药理活性和充分开发利用过山蕨植物资源提供物质基础和科学依据。方法:采用硅胶柱,ODS开放柱,Sephadex LH-20柱以及反相高效液相色谱法对过山蕨水提物进行化学成分的分离。根据其理化性质和波谱数据鉴定化合物结构。结果:分离得到10个化合物,分别鉴定为(7E)-9-hydroxymegastigma-4,7-dien-3-on-9-O-β-D-glucoside (1),bridelionoside F (2),(3R,5S,6S,7E,9S)-megastigman-7-ene-3,5,6,9-tetrol 9-O-β-D-glucopyranoside(3),(6S,7E,9R)-roseoside(4),(3S,5S,6R,9R)-3-hydroxy-5,6-epoxy-β-ionol-9-O-β-glucopyranoside(5),6,9-dihydroxy-4,7-megastigmadien-3-one(6),elaphoside A(7),ptelatoside-A(8),n-butyl-α-β-Dfructofuranoside(9),dibutylphthalate(10)。结论:首次从过山蕨属中分离得到化合物1~10,以上化合物的发现进一步丰富了过山蕨的化学成分组成,为综合开发和利用过山蕨植物资源提供前期的实验基础。Objective: Camptosorus sibiricus is a kind of herbal medicine and famous folk medicine.However,the bioactivities or pharmacological effects of the C.sibiricus remain to be unclear.Therefore,it is necessary to make a systematic study on chemical constituents from C.sibiricus,so as to further study its potential medicinal value,and provide certain chemical basis and foundation for the comprehensive development and the search for pharmacological activity.Method: Various column chromatographic technologies,(silica gel,Sephadex LH-20 and ODS column chromatography) as well as HPLC were adopted to separate chemical constituents of C.sibiricus extract.The structure of the purified compounds was elucidated by nuclear magnetic resonance(NMR) and high-resolution electrospray ionization-mass spectrometry(HR-ESI-MS).Result: Totally 10 compounds have been isolated from water extract of C.sibiricus.By spectroscopic methods,they were elucidated as 7-dien-3-on-9-O-β-D-glucoside(1),bridelionoside F(2),(3 R,5 S,6 S,7 E,9 S)-megastigm,an-7-ene-3,5,6,9-tetrol 9-O-β-D-glucopyranoside(3),(6 S,7 E,9 R)-roseoside(4),(3 S,5 S,6 R,9 R)-3-hydroxy-5,6-epoxy-β-ionol-9-O-β-glucopyranoside(5), 6, 9-dihydroxy-4, 7-megastigmadien-3-one(6),(3 R,6 R,7 E,9 R)-3,9-dihydroxy-4,elaphoside A(7),ptelatoside-A(8),n-butyl-a-β-D-fructofuranoside(9),dibutylphthalate(10) based on physical and chemical properties.Conclusion: All compounds were obtained from C.sibiricus for the first time.The discovery of these compounds further enriched the chemical constituents of C.sibiricus,and provided experimental and scientific basis for the comprehensive development and utilization of C.sibiricus.

关 键 词：过山蕨 水提物 (7E)-9-hydroxymegastigma-4 7-dien-3-on-9-O-β-D-glucoside bridelionoside F 

分 类 号：R289[医药卫生—方剂学] R284.2[医药卫生—中药学]