Pellino 1、锌指蛋白A20、NF-κB P65及IL-6在胎膜早破中的表达及意义  

The expression and significance of Pellino 1, zinc finger protein A20, NF-κB P65 and IL-6 in premature ruptured placental membranes

作　　者：宋春红[1] 甄娟[1] 王建平[1] 杨倩[1] 朱玉芬 高士程 SONG Chun-hong;ZHEN Juan;WANG Jian-ping;YANG Qian;ZHU Yu-fen;GAO Shi-cheng(Department of Pathology,the Fourth Hospital of Shijiazhuang,Shijiazhuang 050011,China)

机构地区：[1]石家庄市第四医院病理科

出　　处：《天津医药》2019年第12期1235-1239,共5页Tianjin Medical Journal

基　　金：河北省“三三三人才工程”资助项目（A2017002093）;河北省医学科学研究重点课题计划(20181063)

摘　　要：目的探讨胎膜早破(PROM)患者胎膜组织Pellino 1、锌指蛋白A20、核因子-κB(NF-κB)P65及羊水白细胞介素-6(IL-6)的表达及其相关性,为深入了解胎膜早破的发生发展机制提供新途径。方法分别选择30例足月胎膜早破(tPROM)、未足月胎膜早破(pPROM)患者及正常妊娠晚期妇女(对照组),应用免疫组化染色法及Western blot检测胎膜组织Pellino 1、锌指蛋白A20、NF-κB P65表达部位及含量,ELISA法测定羊水中IL-6水平,并分析蛋白表达的相关性。结果 PROM组胎膜组织Pellino 1、NF-κB P65及羊水IL-6表达水平高于对照组(P<0.05),胎膜组织锌指蛋白A20表达低于对照组(P<0.05);pPROM组胎膜组织Pellino 1、NF-κB P65表达及羊水IL-6水平高于tPROM组(P<0.05),pPROM组锌指蛋白A20表达低于tPROM组(P<0.05)。胎膜组织中Pellino 1、NF-κB P65表达与羊水IL-6水平呈正相关(P<0.01),锌指蛋白A20表达与羊水IL-6水平呈负相关(P<0.01)。结论 Pellino 1、NF-κB P65及炎性细胞因子IL-6表达水平升高、锌指蛋白A20表达水平降低在胎膜早破发生中发挥重要作用。Objective To investigate the expressions of Pellino 1,zinc finger protein A20(A20) and NF-κB P65 in fetal membranes of patients with premature rupture of the membranes(PROM),analyze their relationship with the level of IL-6 in amniotic fluid,and provide a new way for further understanding the mechanism of the occurrence and development of PROM.Methods Sixty pregnant women with PROM(including 30 with term premature rupture of the fetal membranes,tPROM and 30 with preterm premature rupture of the fetal membranes,pPROM) and 30 women with healthy term pregnancy(control group) were enrolled in the experiment.Immunohistochemistry and Western blot assay were performed to evaluate the expressions of Pellino 1,A20 and NF-κB P65 in fetal membranes.ELISA was used to measure the level of IL-6 in amniotic fluid.The relationship between the expressions of Pellino 1,A20 and NF-κB P65 and the level of IL-6 was analyzed.Results The expression levels of Pellino 1,NF-κB P65 and IL-6 were significantly higher in PROM group than those in control group(P <0.05).On the contrary,the expression of A20 was significantly lower in PROM group than that in control group(P <0.05).The expression levels of Pellino 1,NF-κB P65 and IL-6 were significantly higher in pPROM group than those in tPROM group(P <0.05).And the expression of A20 was significantly lower in pPROM group than that in tPROM group(P <0.05).Furthermore,the expressions of Pellino 1 and NF-κB P65 were positively correlated with the level of IL-6(P <0.01).However,the expression of A20-was negatively correlated with the level of IL-6(P <0.01).Conclusion The over-expressions of Pellino 1,NF-κB P65 and IL-6 and the down-expression of A20 play an important role in PROM.

关 键 词：胎膜早破 肿瘤坏死因子α诱导蛋白3 NF-ΚB 白细胞介素6 Pellino 1 锌指蛋白A20 

分 类 号：R714[医药卫生—妇产科学]