红树林源白骨壤链霉菌中多环稠合大环内酰胺类化合物的结构多样性研究  

Study on polycyclic tetramate macrolactam metabolites from mangrove-derived Streptomyces avicenniae

作　　者：翁静怡 步绪亮 徐俊[2] 徐岷涓[1] Jingyi Weng;Xuliang Bu;Jun Xu;Minjuan Xu(Key Laboratory of Systems Biomedicine,Ministry of Education,Shanghai Center for Systems Biomedicine,Shanghai Jiao Tong University,Shanghai 200240,China;State Key Laboratory of Microbial Metabolism,School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海交通大学系统生物医学教育部重点实验室系统生物医学研究院,上海200240 [2]上海交通大学微生物代谢国家重点实验室生命科学技术学院,上海200240

出　　处：《微生物学报》2020年第1期106-117,共12页Acta Microbiologica Sinica

基　　金：上海交通大学“转化医学交叉研究基金”(ZH2018ZDB08)~~

摘　　要：【目的】通过基因组挖掘的方法,研究红树林来源白骨壤链霉菌Streptomyces avicenniae 9-9中多环稠合大环内酰胺(PTMs)类化合物的结构多样性。【方法】通过生物信息学分析白骨壤链霉菌基因组序列,寻找PTMs类化合物的生物合成相关基因;利用UPLC-MS/MS技术对该菌的次级代谢产物进行分析。【结果】在白骨壤链霉菌基因组中发现PTMs生物合成基因簇(aviA-D);从菌液提取物中鉴定出5个PTMs类化合物,其中包括ikarugamycin(化合物1)和capsimycin B(化合物2);基于PTMs类化合物5-6-5环类型的MS/MS碎裂规律,对化合物3–5的结构进行了推测。【结论】红树林来源白骨壤链霉菌S.avicenniae 9-9具有产生5-6-5环类型的PTMs类化合物的能力。[Objective]Based on genome mining,we analyzed the secondary metabolites from mangrove-derived Streptomyces avicenniae 9-9,targeting on polycyclic tetramate macrolactams(PTMs).[Methods]Bioinformatics analysis was carried out to analyze the genome sequence of Streptomyces avicenniae 9-9.We searched PTMs by the method of high-performance liquid chromatography and mass spectroscopy.[Results]We found four genes,named aviA-D involved in PTMs biosynthetic gene clusters in Streptomyces avicenniae 9-9.Two PTMs,1 and 2,were identified as ikaruganmycin and capsimycin B,respectively.Compound 3 through 5 also belonged to PTMs family,and their structures were proposed based on MS/MS fragmentation pattern.[Conclusion]Mangrove-derived Streptomyces avicenniae 9-9 can produce PTMs compounds with the 5-6-5 fused tricyclic skeleton.

关 键 词：红树林 白骨壤链霉菌 次级代谢产物 多环稠合大环内酰胺 生物合成 

分 类 号：Q936[生物学—微生物学]