血清生长停滞特异性蛋白6c.834+7G>A单核苷酸多态性与重度子痫前期风险增加的相关性  

Correlation study between serum growth arrest-specific protein 6(GAS6)c.834 + 7G>A polymorphisms and increased risk of Severe preeclampsia

作　　者：刘力[1] 张敬蕊 张含 侯晓琳[2] 李瑶 罗茜茜 LIU Li;ZHANG Jingrui;ZHANG Han;HOU Xiaolin;LI Yao;LUO Xixi(Department of Clinical laboratory,the Fourth Hospital of Shijiazhuang,Shijiazhuang 050000,China)

机构地区：[1]石家庄市第四医院检验科,石家庄050000 [2]石家庄市第四医院产科,石家庄050000

出　　处：《实用医学杂志》2020年第2期195-199,205,共6页The Journal of Practical Medicine

基　　金：石家庄市科学技术研究与发展指导计划项目(编号：171462113)

摘　　要：目的检测重度子痫前期患者血清生长停滞特异性蛋白6(GAS6)基因位点c.834+7G>A多态性,评价其与疾病风险增加的相关性及作为生物标志物预警重度子痫前期(SPE)患者的意义。方法563例育龄妇女纳入本研究,SPE组为213例重度子痫前期患者,对照组为350例正常妊娠妇女。采用常规检测收集普通风险指标并比较其在SPE组及对照组之间的差异。采用限制性片段长度多态性聚合酶链反应(PCR⁃RFLP)检测GAS6 c.834+7G>A的基因型,对GAS6单核苷酸多态性资料进行卡方检验,Logistic回归分析计算不同等位基因及基因型的优势比(odds ratio,OR)及95%置信区间(95%CI)。结果SPE组与对照组普通风险指标(如孕周、血小板计数、收缩压、舒张压、血肌酐、尿蛋白和D⁃Dimer等)均存在差异。SPE组GAS6 c.834+7G>A位点等位基因G频率(0.651)高于对照组(0.463),差异有统计学意义(P<0.05);同时与等位基因A相比,等位基因G患病的风险提高2.2倍(OR=2.157,95%CI:1.683~2.766)。SPE组GAS6 c.834+7G>A位点基因型GG频率(0.512)明显高于对照组(0.200),差异有统计学意义(P<0.05);且与等位基因AA相比,基因型GG患病的风险提高3.4倍(OR=3.397,95%CI:2.131~5.416),与等位基因AG相比提高4.8倍(OR=4.775,95%CI:3.143~7.256)。结论SPE组GAS6 c.834+7G>A等位基因及基因型频率与对照组存在差异。等位基因G及基因型GG与重度子痫前期风险增加具有相关性,该位点的基因分型可为重度子痫前期的发病机理研究奠定基础。Objective Serum growth arrest specific protein(GAS)6 c.834+7G>A polymorphism was investigated,its correlation with increased desease risk of Severe preeclampsia(SPE)and its significance as a biomarker for early warning of SPE were evaluated.Methods The case⁃control study consisted of 563 subjects,including 213 patients in SPE group and 350 normal pregnant women.Common risk indicators were collected by conventional tests.The genotyping of GAS6 c.834+7G>A polymorphisms were carried out by polymerase chain reaction⁃restriction fragment length polymorphisms(PCR⁃RFLP)analysis.Chi⁃square tests were used to determine the genotype distributions.Logistic regression analysis was performed to estimate odd ratios(ORs)and 95%confidence intervals(95%CI).Results There were differences between SPE and control groups in common risk indicators such as gestational weeks,platelet count,systolic blood pressure,diastolic blood pressure,serum creati⁃nine,urinary protein and D⁃Dimer.The frequency of GAS6 c.834+7G>A Allele G in SPE(0.651)was higher than that in control group(0.463)(P<0.05).Meanwhile,the risk of Allele G was 2.2⁃fold higher than that of Allele A(OR=2.157,95%CI:1.683~2.766)).The frequency of GAS6 c.834+7G>A genotype GG in SPE group(0.512)was higher than that in control group(0.200)(P<0.05).The risk of genotype GG was 3.4⁃fold higher than that of Genotype AA(OR=3.397,95%CI:2.131~5.416)and 4.8⁃fold than that of Genotype AG(OR=4.775,95%CI:3.143~7.256).Conclusions There were significant difference in the GAS6 c.8347G>A genotype and the allele frequencies between SPE group and control group(P<0.05).Allele G and genotype GG were correlated with increased risk of SPE and the genotype of this site laid the foundation for studying the pathogenesis of SPE.

关 键 词：生长停滞特异性蛋白6 c.834+7G>A位点 重度子痫前期 单核苷酸多态性 

分 类 号：R714.244[医药卫生—妇产科学]