百合抗尖孢镰刀菌细胞突变系的防御酶活性变化  

Changes of Activity of Defense Enzymes in Mutant Cell Lines of Lily Resistant to Fusarium oxysporum

作　　者：张艺萍[1,2] 杨秀梅 许凤[1] 王丽花[1] 苏艳[1] 唐艺蓉[1,2] 瞿素萍 吴学尉[3] ZHANG Yi-ping;YANG Xiu-mei;XU Feng;WANG Li-hua;SU Yan;TANG Yi-rong;QU Su-ping;WU Xue-wei(Flower Research Institute,Yunnan Academy of Agricultural Sciences/National Engineering Research Center for Ornamental Horticulture/Yunnan Flower Breeding Key Lab/Flower Technology Research Center of Yunnan/Yunnan Provincial Key Laboratory of flower Genetic Improvement,Kunming 650205,China;Yunnan Yunke Flower Limited Company,Kunming 650200,China;School of Agriculture,Yunnan University,Kunming 650200,China)

机构地区：[1]云南省农业科学院花卉研究所/国家观赏园艺工程技术研究中心/云南省花卉育种重点实验室/云南省花卉工程技术研究中心/云南省昆明市花卉遗传改良重点实验室,云南昆明650205 [2]云南云科花卉有限公司,云南昆明650200 [3]云南大学农学院,云南昆明650200

出　　处：《江西农业学报》2020年第1期33-37,共5页Acta Agriculturae Jiangxi

基　　金：云南省科技人才和平台计划项目(2017HB083);国家自然科学基金项目(31260484);部省合建项目(2018BSHJ0108)

摘　　要：以研究获得的东方百合品种‘卡萨布兰卡’(‘Casa Blanca’)的抗病无性系和感病无性系为实验材料,利用紫外分光光度计分析了两个品系经尖孢镰刀菌及其毒素诱导后体内POD (peroxidase)(过氧化物酶)、PPO (polyphenol oxidase)(多酚氧化酶)、PAL (phenylalanine ammonia-lyase)(苯丙氨酸解氨酶)、β-1,3-葡聚糖酶、几丁质酶活性的变化。结果表明:经尖孢镰刀菌及其毒素诱导后,东方百合抗病无性系和感病无性系体内的POD、PPO、PAL及几丁质酶的活性都表现出先升高后降低的变化趋势,而β-1,3-葡聚糖酶的活性则表现出逐渐升高的变化趋势;抗病无性系的POD、PPO、PAL、几丁质酶活性均比感病无性系有所提高;抗病无性系的POD、PPO、PAL活性均在处理48 h时达到最高。In the present study, the resistant and susceptible clones of oriental lily variety ‘Casa Blanca’ were used as experimental materials. The change trend of antiviral defense enzyme activity of peroxidase(POD), phenylalanine ammonia enzyme(PAL), polyphenol oxidase(PPO), beta 1, 3 glucanase and chitinase induced by Fusarium oxysporum and its toxin was analyzed by ultraviolet spectrophotometer. The results showed that the activity of POD, PPO, PAL and chitinase of resistant and susceptible clones firstly increased, and then decreased after F. oxysporum and toxin treatment. However, beta 1, 3 glucanase gradually rose. The POD, PPO, PAL, chitinase activity of resistant clones increased more than that of susceptible ones. The POD, PPO, PAL activity of disease-resistant clones reached the highest level at 48 h.

关 键 词：百合 抗尖孢镰刀菌细胞突变系 生化抗性 防御酶 活性 

分 类 号：S436.44[农业科学—农业昆虫与害虫防治]