鞘糖脂的人工合成体系研究进展  

Advances in artificial biosynthesis of glycosphingolipids

作　　者：陈晓辉[1] 张雪 杨广宇[1] CHEN Xiaohui;ZHANG Xue;YANG Guangyu(School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海交通大学生命科学技术学院

出　　处：《生物加工过程》2020年第1期21-26,125,共7页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金(21627812、31670791)

摘　　要：鞘糖脂(glycosphingolipids,GSLs)是真核生物细胞膜的重要组成部分,广泛参与细胞信号传导、免疫、增殖及凋亡等各项生理活动。鞘糖脂类具有作为药物、治疗靶标及潜在诊断标记的潜力,并且与多种疾病的发病机制及相关药物开发密切相关。目前,鞘糖脂类药物的大量制备主要依赖从动物脑组织中提取,存在成本较高、来源有限且有受疯牛病等人畜共患病污染的潜在危险。因此,对鞘糖脂的人工生物合成研究成为学术界的热点。本文中,笔者主要阐述鞘糖脂的化学酶法合成和人工细胞工厂构建方面的研究进展。Glycosphingolipids(GSLs)are important components of eukaryotic cell membrane,and widely involved in various physiological processes such as cell signaling,immunity,proliferation and apoptosis.Glycosphingolipids have potential as diagnostic markers,therapeutic targets or drugs for various diseases,and have attracted extensive attention in pharmaceutical field.Isolation of glycosphingolipids from natural sources is common but subject to several notable limitations,including potential transmission of disease,heterogeneity,and in some cases,scarcity.Therefore,research on the methods for biosynthesis of glycosphingolipids has attracted much attention recently.This review describes the research progress in the chemo-enzymatic synthesis and the construction of cell factories for glycosphingolipids.

关 键 词：鞘糖脂 生物合成 细胞工厂 化学酶法合成 治疗靶标 

分 类 号：TQ463[化学工程—制药化工] Q546[生物学—生物化学]