高效液相色谱法测定3种含香附制剂中α-香附酮含量  

Content Determination of α-Cyperone in Cyperi Rhizoma Preparations by HPLC

作　　者：吴丽 周书来[2] WU Li;ZHOU Shulai(Leshan Food and Drug Inspection Center,Leshan,Sichuan,China 611000;Lesan Vocational&Technical College,Leshan,Sichuan,China 611000)

机构地区：[1]四川省乐山市食品药品检验检测中心,四川乐山614000 [2]乐山职业技术学院,四川乐山614000

出　　处：《中国药业》2020年第5期102-104,共3页China Pharmaceuticals

基　　金：乐山职业技术学院科研项目[KY2018008]

摘　　要：目的建立测定3种含香附制剂(槟榔四消丸、痛经丸和千金止带丸)中α-香附酮含量的高效液相色谱(HPLC)法。方法色谱柱为Welch Ultimate LP-C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.05%磷酸溶液(75∶25,V/V),流速为1.0 m L/min,柱温为30℃,检测波长为249 nm,进样量为10μL。结果槟榔四消丸、痛经丸和千金止带丸中α-香附酮与相邻峰的分离度符合要求,质量浓度线性范围为1.86~74.30μg/m L(r=0.9999),平均加样回收率为98.89%~99.38%,RSD为0.92%~1.65%(n=6),精密度、重复性、溶液稳定性均符合要求。结论该方法简单、快速、准确,可用于以上3种含香附制剂中α-香附酮的含量测定。Objective To establish a high performance liquid chromatography(HPLC)method for the content determination ofα-Cyperone in three Cyperi Rhizoma Preparations.Methods The analysis was performed on a Welch Ultimate LP-C18 column(250 mm×4.6 mm,5μm)with the mobile phase consisting of methanol-0.05%phosphoric acid(75:25)at the flow rate of 1.0 m L/min,the column temperature was 30℃,the detection wavelength was 249 nm,and the injection volume was 10μL.Results In Binlang Sixiao Wan,Tongjin Wan and Qianjin Zhidai Wan,α-Cyperone peak was successfully separated from each other.The linear ranges of calibration curves were 1.86-74.30μg/m L(r=0.9999).The average recoveries were 98.89%-99.38%,and the RSD were 0.92%-1.65%,respectively(n=6).The precision,repeatability and stability were compliance with requirements.Conclusion The established method is simple,fast,and accurate,and can be used for the content determination ofα-Cyperone in three Cyperi Rhizoma Preparations.

关 键 词：香附酮 香附制剂 槟榔四消丸 痛经丸 千金止带丸 高效液相色谱法 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学]