RYR2、FKBP12.6及CaMK-Ⅱ在COPD患者肺组织中的表达研究  

Study on expression of RYR2,FKBP12.6 and CaMK-Ⅱin lung tissue of patients with chronic obstructive pulmonary disease

作　　者：王曦 李萍[2] 耑冰[2] 苑群 谢骏 杨朝 Wang Xi;Li Ping;Zhuan Bing;Yuan Qun;Xie Jun;Yang Zhao(Department of Respiratory Medicine,Suzhou Science&Technology Town Hospital,Suzhou 215153,China;Department of Respiratory Medicine,People's Hospital of Ningxia Hui Autonomous Regin,Yinchuan 750011,China;Department of Cardiothoracic Surgery,Suzhou Science&Technology Touun Hospital,Suzhou 215153,China)

机构地区：[1]苏州科技城医院呼吸内科,215153 [2]宁夏回族自治区人民医院总院呼吸内科,银川750011 [3]苏州科技城医院心胸外科,215153

出　　处：《国际呼吸杂志》2020年第2期94-101,共8页International Journal of Respiration

基　　金：国家自然科学基金(81160011、81560014);宁夏回族自治区2017年度留学人员创新创业项目(宁人社函〔2017〕84号);宁夏回族自治区重点研发计划一般项目(2018BEG03052、2018BEG03054)。

摘　　要：目的探讨兰尼碱(RYR2)、FK506结合蛋白12.6(FKBP12.6)及钙调蛋白依赖性蛋白激酶Ⅱ(CaMK-Ⅱ)在COPD患者肺组织中的表达及意义。方法回顾性分析2012年11月至2017年4月宁夏回族自治区人民医院心胸外科肺癌手术后患者30例,根据入院肺功能、超声心动图检查结果,将患者分为3组:慢性阻塞性肺疾病(COPD)合并肺动脉高压(PH)组、COPD组、非COPD且非PH患者对照组,取手术切除后距癌变区>5 cm的组织。测量3组肺血管组织石蜡切片中肺小动脉面积。用免疫组织化学法分别观察RYR2、FKBP12.6及CaMK-Ⅱ表达,并做相关性分析。结果(1)与对照组比较,COPD合并PH组、COPD组平滑肌面积占血管总面积百分比(WA%)、肺动脉收缩压(PASP)均增高(F=24.115、9.421,P值均<0.05),COPD合并PH组WA%和PASP高于COPD组。(2)与对照组比较,COPD合并PH组、COPD组RYR2、FKBP12.6及CaMK-Ⅱ的表达IOD均增高(F=9.219、6.143、11.337,P值均<0.05),COPD合并PH组RYR2、FKBP12.6及CaMK-Ⅱ的表达IOD高于COPD组。(3)相关性分析提示COPD合并PH组、COPD组RYR2的IOD值与WA%呈正相关(r=0.547、0.771,P值均<0.01),COPD合并PH组RYR2的IOD值与PASP呈正相关(r=0.773,P<0.01)。COPD合并PH组、COPD组FKBP12.6的IOD值与WA%呈正相关(r=0.796、0.810,P值均<0.01),COPD合并PH组FKBP12.6的IOD值与PASP呈正相关(r=0.729,P<0.01)。COPD合并PH组、COPD组CaMK-Ⅱ的IOD值与WA%呈正相关(r=0.879、0.504,P值均<0.01),COPD合并PH组CaMK-Ⅱ的IOD值与PASP呈正相关(r=0.748,P<0.01)。结论FKBP12.6和CaMK-Ⅱ可能在COPD相关的肺动脉增殖中起一定作用。Objective To explore the significance of immunohistochemical expression of Ryanodine receptor 2(RYR2),FK506-binding protein 12.6(FKBP12.6)and Calcium/calmodulin dependent protein kinaseⅡ(CaMK-Ⅱ)in lung tissues from patients with chronic obstructive pulmonary disease(COPD).Methods A retrospective analysis from November 2012 to April 2017 in People′s Hospital of Ningxia Hui Autonomous Regin,lung tissue of 30 patients after lung cancer surgery,according to the results of pulmonary function test and Cardio-Uhrasonography,the patients were divided into three groups:COPD with pulmonary hypertension(PH)(group A)and COPD group(group B),non-COPD,non-PH patients with pulmonary tumor as the negative control group(group C).Take lung tissue about 5 cm from the cancerous area was removed after surgery.The Circum-ferential area of pulmonary valve of the three groups has been observed.To observe RYR2,FKBP12.6 and CaMK-Ⅱby immunohistochemical method respectively,then make correlation analysis.Results(1)Compared with group C,wall area%(WA%),pulmonary artery systolic pressure(PASP)were higher in group A and group B(F=24.115,9.421,both P<0.05),and WA%,PASP were higher in group A than group B.(2)Compared with group C,the immunohistochemical expressions of RYR2,FKBP12.6 and CaMK-Ⅱin group A and group B were all increased(F=9.219,6.143,11.337,all P<0.05),and the immunohistochemical expressions of RYR2,FKBP12.6 and CaMK-Ⅱin group A was higher than group B.(3)Correlation analysis indicated that the immunohistochemical expressions of RYR2 in group A and B was positively correlated with WA%(r=0.547,0.771,both P<0.01),while the immunohistochemical expressions of RYR2 in group A was positively correlated with PASP(r=0.773,P<0.01).Histochemical expressions of FKBP12.6 in group A were positively correlated with WA%(r=0.796,0.810,both P<0.01),and the immunohistochemical expressions of FKBP12.6 in group A was positively correlated with PASP(r=0.729,P<0.01).The immunohistochemical expressions of CaMK-Ⅱin group A and B were positivel

关 键 词：肺疾病.慢性阻塞性 高血压 肺性 兰尼碱受体钙释放通道 钙-钙调蛋白依赖性蛋白激酶2 FK5O6结合蛋白12.6 

分 类 号：R563[医药卫生—呼吸系统] R734[医药卫生—内科学]