不同培养基和基质对大别山冬青组培苗生根的影响  

Effects of Different Culture Medium and Matrix on Rooting in Ilex dabieshanensis

作　　者：方宇鹏[1] 汪诗德[1] FANG Yupeng;WANG Shide(Garden Department,Chizhou Vocational and Technical College,Chizhou 247000,China)

机构地区：[1]池州职业技术学院园林系

出　　处：《安庆师范大学学报（自然科学版）》2020年第1期98-100,115,共4页Journal of Anqing Normal University(Natural Science Edition)

基　　金：安徽高校自然科学研究重点项目（KJ2017A729）;安徽高等学校质量工程项目-园艺专业教学团队（2018jxtd055）

摘　　要：以大别山冬青(Ilex dabieshanensis)组培苗为试验材料,探索不同培养基及基质对组培苗生根及移栽成活率的影响。研究结果表明:(1)最适宜大别山冬青生根的培养基为1/2 MS培养基;(2)最适宜大别山冬青生根培养的生长调节剂配比为1/2MS+萘乙酸(NAA)0.3 mg·L^-1+吲哚丁酸(IBA)0.2 mg·L^-1,生根率高达95%;(3)最佳移栽基质是蛭石、珍珠岩、泥炭土体积比为2:1:2的基质,控制环境条件,大别山冬青生长状况良好,成活率可达85%。Taking young stem segments of axillary buds of Ilex dabieshanensis as explant materials,the effects of different mediums and substrates on rooting and survival rate of Ilex dabieshanensis were studied.The research results show that the optimal culture medium for rooting was 1/2 MS.The best ratio of growth regulator for rooting was MS+NAA 0.3 mg·L^-1+IBA 0.2 mg·L^-1,the rooting rate was 95%.The best transplanting substrate is a matrix with a volume ratio of vermiculite,perlite,and peat soil of 2:1:2,and the survival rate of plantlets can reach 85%if controlling environmental conditions.

关 键 词：大别山冬青 培养基 基质 生根 

分 类 号：S688[农业科学—观赏园艺]