苏州市RhD阴性献血者血型抗原及表型分布研究  

Distribution of phenotype and blood type antigen in RhD negative blood donors in Suzhou

作　　者：何红红 汤龙海[1] 陆荣[1] 潘志荣[1] 金一鸣[1] 周晓华[1] 王明元[1] HE Honghong;TANG Longhai;LU Rong;PAN Zhirong;JIN Yiming;ZHOU Xiaohua;WANG Mingyuan(Department of Clinical Laboratory,Suzhou Blood Center,Suzhou,215006,China)

机构地区：[1]苏州市中心血站检验科

出　　处：《临床血液学杂志（输血与检验）》2019年第6期946-949,共4页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

基　　金：苏州市科技计划项目合同（No:SYS201664);2017年度苏州市第十九批产业技术创新专项（民生科技）(No:SS201750)

摘　　要：目的:分析苏州地区Rh阴性血型抗原分布特点及表现型规律,为临床输血提供理论依据。方法:选取苏州市中心血站2015-2018年无偿献血者344 367例,运用盐水试管法初筛为RhD阴性样本869例,再用抗人球蛋白微柱凝胶卡式法对其确认,用盐水试管法进行C、c、E、e抗原检测以确定表型。结果:初筛869例RhD阴性标本中有844例标本(97.12%,844/869)确证阴性,占本地区献血者0.25%(844/344 367);D变异型25例(2.88%,25/869),占本地区献血者0.007%(25/344 367),其中以ccEe频率最高(40%,10/25);RhD阴性样本中以ccee、Ccee表现型居多,占比56.40%(476/844)、32.70%(276/844);e、c的抗原频率最高,分别为98.93%(835/844)、95.85%(809/844);RhD阴性献血者主要为A、O、B血型,AB型最少。结论:苏州地区RhD阴性献血者主要表现型为ccee、Ccee,D变异型中ccEe频率最高。对RhD阴性血液样本的定型及对D变异型的筛查,有助于保障本地区临床输血安全,为苏州市稀有血型库的建立提供参考依据。Objective:To investigate the characteristic and distribution of phenotype and blood type antigen in RhD negative blood donors in Suzhou,and provide a theoretical basis for clinical blood transfusion. Method:869 blood samples of RhD negative were screened among the 344 367 blood donors from 2015 to 2018 in Suzhou blood center,and confirmed by the anti-human globulin microcolumn gel card and serologic blood tests for phenotypes. Result:869 RhD negative specimens were confirmed to be RhD negative 844(97.12%,844/869),accounting for 0.25%(844/344 367)of the donors in the region;D variant phenotypes 25(2.88%,25/869),accounting for 0.007%(25/344 367),ccEe(40%,10/25)had the highest frequency.The RhD negative were mostly ccee and Ccee phenotypes,accounting for 56.40%(476/844) and 32.70%(276/844),and the e and c antigen occupied higher antigen frequencies,98.93%(835/844)and 95.85%(809/844)respectively.RhD negative phenotypes were mostly A,O,B types.Conclusion:The main phenotypes of RhD negative blood donors in Suzhou were ccee and Ccee,and the ccEe frequency was the highest in D variant.The characteristic of RhD negative blood samples and the screening of D variant would help to ensure the safety of clinical blood transfusion and provide reference for the establishment of rare blood bank in Suzhou.

关 键 词：RHD阴性 D变异型 RhD表现型 稀有血型库 

分 类 号：R457.1[医药卫生—治疗学]