不同贮藏方法对华顶杜鹃花粉活力的影响  

Effects on Pollen Viabilities of Rhododendron huadingense with Different Storage Methods

作　　者：李修鹏 沈登锋 徐沁怡 章建红 何立平 李东宾 余敏芬 沈波 LI Xiu-peng;SHEN Deng-feng;XU Qin-yi;ZHANG Jian-hong;HE Li-ping;LI Dong-bin;YU Min-feng;SHEN Bo(Ningbo Technology Extension Center for Forestry and Specialty Forest Products,Ningbo 315012,Zhejiang,China;Ningbo Agricultural Science Academy,Ningbo 315040,Zhejiang,China;Ningbo forest farm,Yuyao 315440,Zhejiang,China)

机构地区：[1]宁波市林特科技推广中心,浙江宁波315012 [2]宁波市农业科学研究院,浙江宁波315040 [3]宁波市林场,浙江余姚315440

出　　处：《浙江林业科技》2019年第5期72-76,共5页Journal of Zhejiang Forestry Science and Technology

基　　金：宁波市农业重大科技专项(编号：2014C11002)

摘　　要：研究了华顶杜鹃Rhododendron huadingense花粉活力水平及不同贮藏方法对其活力的影响。结果表明,新鲜华顶杜鹃花粉的萌发率高达98.1%;室温存放的新鲜花粉在3 d内可保持较高的活力,3 d后其活力下降迅速;4℃贮藏7 d后的花粉萌发率仍保持在85.5%的较高水平,贮藏15 d时的萌发率仍高于50%,可作为华顶杜鹃花粉的短期(15 d内)保存方法;-20℃贮藏40 d后的花粉萌发率仍能达到87.4%,基本可满足实际需求;-80℃下贮藏60 d后萌发率依旧维持在95.2%,可用于华顶杜鹃花粉的长期保存。本研究结果说明花粉活力不是导致华顶杜鹃濒危的生物学原因。The pollen viability of Rh. huadingense and the effects of different storage methods were studied, the results showed that the germination rate of fresh pollens was as high as 98%, fresh pollens which were stored at room temperature(20℃) had a high activity in 3 days, then the activity of pollens declined rapidly, the germination rate was about 85.5% when the pollens were stored at 4℃ for a week, after half a month,the germination rate was up to 50%., It can be used as a short-term(within 15 days) preservation method for Rh. huadingense pollen. When stored at-20℃ for 40 days, the germination rate of pollens was up to 87.4%, while stored at-80℃ for 60 days, the germination rate could remain at 95.2%, it could be used for the long-term preservation of the pollen. Our results indicate that pollen viability is not the biological reason for the endangered state of Rh. huadingense.

关 键 词：华顶杜鹃 花粉活力 贮藏方法 

分 类 号：S685.21[农业科学—观赏园艺]