清胰汤对大鼠重症急性胰腺炎促炎和抗炎因子等的影响  

Effect of Qingyi Decoction on Pro-inflammatory and Anti-inflammatory Factors in Rats with Severe Acute Pancreatitis

作　　者：肖彦[1] 刘丽蕾[1] 雷衍军[1] 成伟[1] 张兴文[1] 李想[1] 王湘英[1] XIAO Yan;LIU Li-lei;LEI Yan-jun(First department of critical care medicine,hunan provincial people's hospital,changsha 410008)

机构地区：[1]湖南省人民医院重症医学一科,湖南长沙410008

出　　处：《医学临床研究》2019年第12期2289-2291,共3页Journal of Clinical Research

基　　金：湖南省卫生厅中医药科研基金(课题编号:06103)。

摘　　要：【目的】探讨清胰汤对重症急性胰腺炎(SAP)大鼠血清白介素-6(IL-6)、IL-10、肿瘤坏死因子-α(TNF-α)、淀粉酶(AMY)、钙离子(Ca^2+)以及胰腺组织病理评分的影响。【方法】将30只健康SD大鼠随机分为假手术组(SO组)、SAP组及SAP清胰汤治疗组(QYT组),每组10只。SO组:仅开腹翻动胰腺后关腹;用5%牛磺胆酸钠经胰管逆行注射(1 mg/kg)构造SAP模型。建模后24 h三组大鼠均进行再次开腹,观察其腹水、腹腔内皂化斑及胰腺大体形态改变,测定大鼠血清AMY、Ca^2+、TNF-α、IL-6及IL-10水平,并对胰腺组织进行病理学评分。【结果】SAP组可见全胰腺充血水肿、坏死及片状出血;QYT组大鼠可见全胰腺充血水肿,点状出血及坏死灶,SO组大鼠未见上述改变。清胰汤组血清TNF-α、IL-6、IL-10、AMY值及病理学评分明均显著低于SAP组(P<0.05),但高于假手术组(P<0.05)。清胰汤组的血清钙离子浓度显著高于SAP组(P<0.05),低于SO组(P<0.05)。【结论】以清胰汤经胃灌注SAP大鼠可降低血清TNF-α、IL-6、IL-10炎症因子的释放,减轻胰腺组织的出血、坏死,进而起到一定的治疗作用。【Objective】To investigate the effect of Qingyi Decoction on serum interleukin-6(IL-6),IL-10,tumor necrosis factor-α(TNF-α),amylase(Amy),calcium ion(Ca^2+)and pancreatic histopathological score in rats with severe acute pancreatitis(SAP).【Methods】Thirty healthy SD rats were randomly divided into sham operation group(SO Group),SAP Group and SAP Qingyi Decoction treatment group(QYT Group).In the SO Group,the model of sap was established by retrograde injection of 5%sodium taurocholate(1 mg/kg).24 hours after the model was established,three groups of rats were re opened to observe the ascites,saponification spots in the abdominal cavity and the general morphological changes of the pancreas.The serum levels of Amy,Ca^2+,TNF-α,IL-6 and IL-10 were measured,and the pathological scores of the pancreas were made.【Results】The SAP Group showed hyperemia,edema,necrosis and lamellar hemorrhage of the whole pancreas;the QYT Group showed hyperemia,edema,punctate hemorrhage and necrosis of the whole pancreas,the SO Group did not see the above changes.Serum TNF-α,IL-6,IL-10,Amy and pathological scores in the Qingyi Decoction Group were significantly lower than those in the SAP Group(P<0.05),but higher than those in the so Group(P<0.05).The serum calcium concentration of the Qingyi Decoction Group was significantly higher than that of the SAP Group(P<0.05),and lower than that of the SO Group(P<0.05).【Conclusion】Qingyi decoction can reduce the release of TNF-α,IL-6,IL-10 inflammatory factors in serum,reduce the hemorrhage and necrosis of pancreatic tissue,and play a certain therapeutic role.

关 键 词：胰腺炎 急性坏死性 大鼠 

分 类 号：R657.5[医药卫生—外科学]